FastQCFastQC Report
Sun 21 May 2023
EGAF00007838200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53602
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17883.335696429237715No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16363.052124920711914No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT14312.669676504608037No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14172.643558076191187No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11752.1920823849856346No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11232.0950710794373344No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10451.9495541211148837No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10211.9047796724002835No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9761.8208275810604084No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9621.7947091526435581No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9441.761128316107608No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9211.7182194694227826No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8901.6603858064997576No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8291.546584082683482No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6941.2947278086638558No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6561.2238349315324055No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6521.2163725234133056No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5861.093242789448155No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5811.0839147792992798No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5501.0260811163762547No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5481.0223499123167046No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5431.0130219021678295No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5290.9869034737509795No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4980.9290698108279541No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4940.9216074027088541No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4830.901085780381329No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4820.8992201783515541No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4810.8973545763217791No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4530.8451177194880788No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4480.8357897093392038No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4160.7760904443864035No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4060.7574344240886534No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3700.6902727510167531No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3560.664154322599903No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3350.6249766799746278No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3340.6231110779448528No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3290.6137830677959777No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3220.6007238535875526No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3160.5895302414089026No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3130.5839334353195776No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3060.5708742211111526No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3030.5652774150218276No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3030.5652774150218276No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3020.5634118129920526No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2980.5559494048729524No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2940.5484869967538525No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2920.5447557926943024No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2840.5298309764561024No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.5242341703667773No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2800.5223685683370023No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2640.49251893586060225No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT2580.48132532368195213No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2550.4757285175926272No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2340.436550874967352No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2310.43095406887802695No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2250.41976045669937684No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.4048356404611768No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2010.37498600798477666No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1980.36938920189545166No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.3619267937763516No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.3376739673892765No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1810.3376739673892765No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1800.3358083653595015No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.3246147531808515No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.32274915115107644No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.3190179470915264No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1680.3134211410022014No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1660.3096899369426514No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1650.30782433491287636No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1650.30782433491287636No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1620.30222752882355136No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.3003619267937763No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1600.29849632476400134No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1600.29849632476400134No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1570.29289951867467634No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1550.28916831461512627No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1540.2873027125853513No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.28357150852580126No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1410.2630498861982762No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1380.2574530801089512No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1380.2574530801089512No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1370.25558747807917614No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1360.25372187604940116No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1350.2518562740196261No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1340.24999067198985112No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1330.2481250699600761No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.23506585575165106No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1260.23506585575165106No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.23133465169210105No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1240.23133465169210105No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1240.23133465169210105No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.229469049662326No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.222006641543226No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1160.216409835453901No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.21454423342412596No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.21267863139435095No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1120.20894742733480096No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1120.20894742733480096No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1110.20708182530502595No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1100.20521622327525094No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1090.2033506212454759No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.1977538151561509No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1050.1958882131263759No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1050.1958882131263759No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.19402261109660088No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1030.19215700906682587No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1010.18842580500727585No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.18656020297750084No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1000.18656020297750084No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.18469460094772583No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA970.1809633968881758No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT960.1790977948584008No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA960.1790977948584008No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT950.17723219282862582No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.17350098876907577No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA930.17350098876907577No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA910.16976978470952575No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG900.16790418267975074No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA880.16417297862020072No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.16417297862020072No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC860.16044177456065073No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA850.1585761725308757No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA840.1567105705011007No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA840.1567105705011007No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA820.1529793664415507No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG810.15111376441177568No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT800.14924816238200067No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA770.14365135629267564No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.14178575426290063No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA750.13992015223312562No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA750.13992015223312562No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC740.13805455020335064No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT740.13805455020335064No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT730.1361889481735756No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC730.1361889481735756No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA720.1343233461438006No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.1324577441140256No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT710.1324577441140256No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.13059214208425057No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.13059214208425057No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.13059214208425057No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.12686093802470058No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.12312973396515056No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT650.12126413193537554No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA640.11939852990560053No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT640.11939852990560053No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT630.11753292787582553No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC630.11753292787582553No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.11566732584605052No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA610.11380172381627553No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.1100705197567255No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG590.1100705197567255No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT580.1082049177269505No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT570.10633931569717547No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.10447371366740048No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC560.10447371366740048No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG550.10260811163762547No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT540.10074250960785044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)