FastQCFastQC Report
Sun 21 May 2023
EGAF00007838211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48158
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22434.657585447900661No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19574.063706964574941No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT17593.6525603222725196No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12432.5810872544540886No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11322.3505959549815194No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11272.3402134640142864No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10962.2758420200174427No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9161.9020723451970596No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9031.8750778686822542No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8501.7650234644295861No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7531.5636031396652685No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7081.4701607209601726No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6491.347647327546825No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6001.245898916067943No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5961.2375929232941567No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5281.0963910461397899No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5231.086008555172557No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4931.02371360936916No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4871.0112546202084804No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4780.9925661364674612No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4770.9904896382740147No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4770.9904896382740147No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4590.9531126707919764No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4490.9323476888575106No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4330.8991237177623657No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4230.8783587358278997No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4220.8762822376344532No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4160.8638232484737739No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4110.8534407575065409No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4000.8305992773786287No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC3920.8139872918310561No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3910.8119107936376095No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3830.795298808090037No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3820.7932223098965904No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3780.7849163171228042No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3780.7849163171228042No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3600.7475393496407658No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3450.7163918767390672No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3120.6478674363553304No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.6125669670667386No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2870.595954981519166No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.5834959923584866No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2400.49835956642717716No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2400.49835956642717716No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2360.490053573653391No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2270.4713650899123718No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2250.4672120935254786No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.4672120935254786No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2240.465135595332032No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2210.4589061007516923No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2120.44021761701067325No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2080.43191162423688695No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.4298351260434404No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2060.42775862784999374No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2000.41529963868931435No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2000.41529963868931435No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1950.4049171477220815No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1950.4049171477220815No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.3945346567548486No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1890.3924581585614021No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1860.3862286639810623No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1840.38207566759416917No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1730.3592341874662569No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1700.3530046928859172No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1670.34677519830557746No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1620.3363927073383446No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1560.3239337181776652No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1540.31978072179077205No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.3093982308235392No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1460.30316873624319945No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.30316873624319945No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1450.3010922380497529No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1440.29901573985630636No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.29693924166285973No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1380.2865567506956269No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1320.27409776153494747No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1260.261638772374268No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1250.2595622741808215No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1220.25333277960048173No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.24710328502014203No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.23879729224635574No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1130.23464429585946261No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1120.232567797666016No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1110.23049129947256947No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1090.22633830308567632No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1070.22218530669878317No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1070.22218530669878317No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.22010880850533662No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.21803231031189002No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1020.21180281573155033No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1010.20972631753810372No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1010.20972631753810372No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA960.19934382657087085No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA960.19934382657087085No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT960.19934382657087085No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.19311433199053116No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.19311433199053116No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC930.19311433199053116No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA920.19103783379708458No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT920.19103783379708458No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT880.1827318410232983No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT870.18065534282985174No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA850.1765023464429586No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC830.17234935005606544No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT810.1681963536691723No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT800.16611985547572572No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.16611985547572572No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA780.1619668590888326No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT770.15989036089538602No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.15781386270193945No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA760.15781386270193945No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.15781386270193945No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT750.15573736450849288No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT740.1536608663150463No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT720.14950786992815318No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA720.14950786992815318No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA710.1474313717347066No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC700.14535487354126003No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA690.14327837534781346No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC690.14327837534781346No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA680.14120187715436688No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.14120187715436688No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC680.14120187715436688No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT670.1391253789609203No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT670.1391253789609203No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.1391253789609203No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.13704888076747374No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA660.13704888076747374No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.13704888076747374No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.1328958843805806No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA630.130819386187134No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT630.130819386187134No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.12874288799368744No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC620.12874288799368744No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT610.12666638980024086No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.12666638980024086No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA610.12666638980024086No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA610.12666638980024086No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC600.12458989160679429No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.12043689521990117No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC580.12043689521990117No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.12043689521990117No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.1183603970264546No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT570.1183603970264546No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA570.1183603970264546No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT570.1183603970264546No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA560.116283898833008No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA550.11420740063956143No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA550.11420740063956143No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA550.11420740063956143No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCT540.11213090244611486No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT540.11213090244611486No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG540.11213090244611486No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.11213090244611486No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC530.11005440425266831No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT530.11005440425266831No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT520.10797790605922174No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC510.10590140786577516No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT500.10382490967232859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)