FastQCFastQC Report
Sun 21 May 2023
EGAF00007838213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47002
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT34397.316709927237139No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG25495.42317348197949No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT21834.644483213480277No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG19004.042381175269137No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG17363.6934598527722224No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA15013.1934811284626186No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA12002.5530828475384024No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11732.4956384834687886No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11702.4892557763499426No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10522.238202629675333No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9992.12544147057572No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA9842.09352793498149No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT9652.0531041232287985No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9572.036083570911876No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8381.7829028551976513No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7331.559508106038041No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7301.5531253989191949No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6831.4531296540572742No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6551.3935577209480448No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6391.3595166163141994No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6301.3403684949576615No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6291.3382409259180459No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5951.2659035785711248No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5041.072294795966129No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4931.0488915365303604No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3950.8403897706480576No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3820.8127313731330582No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.6552912642015234No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.63401557380537No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2930.6233777286072933No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2920.621250159567678No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2900.6169950214884473No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2720.5786987787753712No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.5744436406961406No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.5318922599038339No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2480.5276371218246032No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.49785115526998847No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2210.47019275775498914No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2210.47019275775498914No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2200.4680651887153738No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2110.44891706735883574No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2070.44040679120037446No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2060.43827922216075915No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1890.40211054848729844No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1820.38721756520999107No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.38083485809114503No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.38083485809114503No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1750.37232458193268375No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.36168673673460705No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1570.3340283392196077No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1550.329773201140377No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1530.32551806306114633No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.32126292498191567No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1500.3191353559423003No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1480.3148802178630697No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1450.30849751074422366No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1410.2999872345857623No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.2999872345857623No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.2872218203480703No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1340.28509425130845495No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1250.26594612995191697No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1240.2638185609123016No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1100.2340325943576869No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1060.22552231819922558No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.2212671801199949No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA980.2085017658823029No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.20637419684268757No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA960.20424662780307223No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.19999148972384154No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA880.18722607548614953No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT860.18297093740691886No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA810.1723330922088422No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA790.1680779541296115No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT790.1680779541296115No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT780.16595038508999618No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA770.16382281605038085No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.16382281605038085No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.15956767797115015No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA750.15956767797115015No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.1553125398919195No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.15318497085230415No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA710.1510574018126888No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.14892983277307348No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA690.14680226373345814No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.14254712565422747No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT650.1382919875749968No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC650.1382919875749968No Hit
ATCCTACATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.13403684949576614No Hit
ATCCTACATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.1319092804561508No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT600.12765414237692013No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA600.12765414237692013No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.12127143525807413No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.1191438662184588No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.1191438662184588No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.1191438662184588No Hit
CTGATCCATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA550.11701629717884345No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA540.11488872813922811No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT540.11488872813922811No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.11063359005999746No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC510.1085060210203821No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.1085060210203821No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT510.1085060210203821No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT500.10637845198076677No Hit
CTGATCCATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA500.10637845198076677No Hit
CTGATCCATGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA490.10425088294115145No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.10425088294115145No Hit
CTGATCCATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA480.10212331390153612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)