FastQCFastQC Report
Sun 21 May 2023
EGAF00007838215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48133
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21614.489643280078117No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT16473.4217688488147426No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT15253.16830448964328No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11992.4910144807097003No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11132.312342883260964No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10952.274946502399601No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10432.1669125132445517No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10312.1419815926703096No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9481.969542725365134No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7261.5083206947416534No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG7191.4937776577400121No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6231.2943302931460743No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5671.177985997132944No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5551.153055076558702No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5491.1405896162715807No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5441.13020173269898No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5431.1281241559844597No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5391.119813849126379No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4971.0325556271165313No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4841.005547129827769No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4831.0034695531132487No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4670.9702283256809257No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4590.9536077119647643No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4580.9515301352502441No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4290.8912804105291587No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4280.8892028338146386No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.8829701036710781No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3990.8289531090935532No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3950.8206428022354726No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA3840.7977894583757505No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3720.7728585378015084No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT3710.7707809610869881No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3620.7520827706563065No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.7084536596513826No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3340.6939106226497413No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3080.6398936280722166No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3070.6378160513576964No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3020.6274281677850955No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2880.5983420937818129No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2790.5796439033511313No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2780.5775663266366111No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2730.5671784430640102No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2730.5671784430640102No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.56510086634949No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2720.56510086634949No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2640.5484802526333284No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2590.5380923690607275No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2480.5152390252010056No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2480.5152390252010056No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2460.5110838717719651No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT2450.509006295057445No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2330.4840753744832028No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2320.4819977977686826No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2250.46745476076704134No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2000.41551534290403674No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1860.3864292689007542No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1820.3781189620426734No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1780.36980865518459266No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1770.3677310784700725No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1750.36357592504103214No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1730.35942077161199176No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1630.3386450044667899No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.3386450044667899No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.3344898510377496No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1590.3303346976087092No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1580.328257120894189No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.328257120894189No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1570.3261795441796688No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1570.3261795441796688No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.3220243907506285No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1500.31163650717802754No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.30748135374898716No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1470.30540377703446697No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1370.2846280098892652No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.2804728564602248No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.2763177030311844No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1300.2700849728876239No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1250.25969708931502294No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1230.25554193588598256No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1190.24723162902790188No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1170.24307647559886147No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1160.2409988988843413No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.2409988988843413No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1150.23892132216982112No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1140.23684374545530096No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1120.23268859202626055No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCT1110.23061101531174039No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT1080.22437828516817981No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1070.22230070845365965No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.22230070845365965No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1060.22022313173913946No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.2181455550246193No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1040.2160679783100991No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.20983524816653853No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1010.20983524816653853No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1000.20775767145201837No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.203602518022978No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT980.203602518022978No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT940.19529221116489726No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT910.1890594810213367No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT870.18074917416325598No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT870.18074917416325598No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT870.18074917416325598No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT860.17867159744873579No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA860.17867159744873579No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT840.17451644401969543No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.17243886730517524No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT790.1641285604470945No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA780.16205098373257432No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.16205098373257432No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA770.15997340701805415No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA770.15997340701805415No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG770.15997340701805415No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT760.15789583030353396No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT750.15581825358901377No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC750.15581825358901377No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.15581825358901377No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.15166310015997342No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT720.14958552344545323No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.14750794673093304No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC710.14750794673093304No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.14750794673093304No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA700.14543037001641285No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT700.14543037001641285No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCATGCT690.14335279330189266No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT670.1391976398728523No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT660.1371200631583321No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA650.13504248644381195No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.13504248644381195No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA640.13296490972929176No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT640.13296490972929176No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA610.1267321795857312No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.1267321795857312No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA610.1267321795857312No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA590.12257702615669083No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA580.12049944944217066No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT580.12049944944217066No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT570.11842187272765048No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT570.11842187272765048No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT570.11842187272765048No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT560.11634429601313027No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.11634429601313027No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA560.11634429601313027No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC540.11218914258408991No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.10803398915504955No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC510.10595641244052936No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA500.10387883572600919No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA490.101801259011489No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA490.101801259011489No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC490.101801259011489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)