FastQCFastQC Report
Sun 21 May 2023
EGAF00007838220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44141
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12062.732153779932489No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11862.686844430348202No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11542.6143494710133433No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9012.0411861987721167No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8892.0140005890215447No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8561.9392401622074715No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7931.7965157110169685No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7671.7376135565573956No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7191.6288711175551074No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6471.4657574590516753No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6441.4589610566140323No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5291.1984322965043837No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5011.1349992070863824No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4711.0670351827099522No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4681.0602387802723092No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4661.0557078453138806No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4581.0375841054801658No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4410.9990711583335221No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4360.9877438209374504No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4300.9741510160621645No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4280.9696200811037358No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4280.9696200811037358No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4160.9424344713531638No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4160.9424344713531638No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4080.9243107315194491No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4040.9152488616025917No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3970.8993905892480913No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3790.8586121746222333No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3640.8246301624340183No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.8200992274755896No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3510.7951790852042319No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3410.7725244104120885No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3330.7544006705783739No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2990.6773747762850865No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2970.6728438413266578No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.6660474388890147No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2920.661516503930586No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2880.6524546340137287No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2870.6501891665345144No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2840.6433927640968714No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.641127296617657No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2820.6388618291384427No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2620.593552479554156No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2620.593552479554156No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2610.5912870120749416No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2590.586756077116513No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2590.586756077116513No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2560.57995967467887No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2400.5437121950114406No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.5301193901361546No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2240.5074647153440112No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2180.4938719104687252No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2170.49160644298951084No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2140.4848100405518679No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2080.4712172356765819No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2070.4689517681973675No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2030.4598898982805102No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1950.44176615844679545No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1940.4395006909675812No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1880.42590788609229513No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1840.41684601617543776No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA1810.4100496137377948No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.40098774382093744No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1670.3783330690287941No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1650.37380213407036544No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.37380213407036544No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1620.3670057316327224No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1610.3647402641535081No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1600.3624747966742937No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1590.3602093291950794No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1580.35794386171586506No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1570.3556783942366507No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1550.35114745927822205No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1530.3466165243197934No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1490.337554654402936No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1480.3352891869237217No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1400.317165447090007No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1390.3148999796107927No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA1370.31036904465236403No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.31036904465236403No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1350.3058381096939353No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.2922453048186493No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1270.28771436986022064No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA1250.283183434901792No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1250.283183434901792No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1250.283183434901792No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1250.283183434901792No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1230.2786524999433633No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1190.26959063002650596No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1180.2673251625472916No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.2537323576720056No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.2514668901927913No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1080.2446704877551483No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1050.23787408531750526No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1050.23787408531750526No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.23560861783829096No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.23107768287986227No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA970.2197503454837906No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA940.21295394304614756No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA940.21295394304614756No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT940.21295394304614756No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA940.21295394304614756No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT940.21295394304614756No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT910.20615754060850458No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT910.20615754060850458No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA890.2016266056500759No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.19936113817086154No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA870.1970956706916472No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT860.19483020321243288No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.19256473573321856No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA840.1902992682540042No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT840.1902992682540042No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT840.1902992682540042No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC810.1835028658163612No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT790.17897193085793253No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT780.1767064633787182No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA780.1767064633787182No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA770.17444099589950388No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT750.1699100609410752No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA730.1653791259826465No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA720.16311365850343218No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT710.16084819102421785No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG700.1585827235450035No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC700.1585827235450035No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.15631725606578917No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA680.15405178858657484No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA670.1517863211073605No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT660.14952085362814618No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA660.14952085362814618No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.14272445119050317No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCT630.14272445119050317No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT620.14045898371128882No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.1381935162320745No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC600.13592804875286016No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT600.13592804875286016No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC600.13592804875286016No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT590.1336625812736458No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA580.13139711379443147No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.13139711379443147No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT580.13139711379443147No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA580.13139711379443147No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.12913164631521715No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.12913164631521715No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA570.12913164631521715No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCAAACT570.12913164631521715No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT560.1268661788360028No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC560.1268661788360028No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT550.12460071135678846No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.12233524387757415No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC540.12233524387757415No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC530.1200697763983598No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG520.11780430891914548No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT520.11780430891914548No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.11780430891914548No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC510.11553884143993114No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC510.11553884143993114No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC500.1132733739607168No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG500.1132733739607168No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA500.1132733739607168No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA490.11100790648150245No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA490.11100790648150245No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT480.10874243900228812No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG470.10647697152307378No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA460.10421150404385944No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA460.10421150404385944No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.10194603656464511No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT450.10194603656464511No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT450.10194603656464511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)