FastQCFastQC Report
Sun 21 May 2023
EGAF00007838226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44386
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12782.8792862614337853No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11772.651737034199973No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11192.521065200738972No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG10682.406164105799126No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10572.381381516694453No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG10102.275492272338125No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10012.255215608525211No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9152.061460820979588No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8611.9398008381021041No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7881.7753345649529133No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG7341.6536745820754293No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7281.640156806200153No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7161.6131212544496014No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT6411.4441490560086514No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6271.4126075789663408No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5871.3224890731311676No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5511.2413824178795116No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5471.2323705672959941No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5361.2075879781913217No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5191.169287613211373No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4621.040868742396251No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4611.0386157797503717No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4611.0386157797503717No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4511.0160861532915784No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4300.9687739377281126No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4250.9575091244987158No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4210.9484972739151986No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4190.9439913486234398No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.9439913486234398No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4130.9304735727481639No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3770.849366917496508No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3760.8471139548506287No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3470.781778038120128No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3350.754742486369576No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3350.754742486369576No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3260.734465822556662No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.6894065696390753No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2830.6375884287838507No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2770.6240706529085748No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2750.6195647276168161No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2740.6173117649709368No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2670.6015410264497815No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2650.5970351011580227No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2640.5947821385121435No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2570.5790113999909882No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2550.5745054746992295No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2370.5339521470734016No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2370.5339521470734016No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2370.5339521470734016No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2310.5204343711981255No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2270.5114225206146082No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT2170.4888928941558149No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2140.48213400621817687No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2120.47762808092641823No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2100.47312215563465954No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2090.4708691929887802No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2060.4641103050511423No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1710.38525661244536563No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1690.380750687153607No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1600.360474023340693No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1580.3559680980489343No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.3559680980489343No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1540.34695624746541703No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.340197359527779No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1460.32893254629838237No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1450.32667958365250305No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1420.31992069571486503No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1410.3176677330689857No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1400.3154147704231064No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1360.30640291983958906No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1360.30640291983958906No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1360.30640291983958906No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1320.2973910692560717No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1310.2951381066101924No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.2951381066101924No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.2883792186725544No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.28162033073491644No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1240.2793673680890371No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1240.2793673680890371No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1220.2748614427972784No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1210.27260848015139905No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.2681025548596404No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1190.2681025548596404No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.26359662956788177No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1080.24331996575496778No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.24331996575496778No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1020.22980218987969178No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.2275492272338125No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1010.2275492272338125No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1000.22529626458793311No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT970.21853737665029513No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT970.21853737665029513No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT970.21853737665029513No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA970.21853737665029513No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.21177848871265717No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC930.2095255260667778No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT920.2072725634208985No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT910.20501960077501913No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA910.20501960077501913No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT880.19826071283738114No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA870.19600775019150185No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA830.18699589960798452No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.18699589960798452No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.18699589960798452No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.18248997431622582No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT810.18248997431622582No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA790.17798404902446716No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC780.17573108637858786No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT780.17573108637858786No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT780.17573108637858786No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT780.17573108637858786No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT760.1712251610868292No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.1712251610868292No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT760.1712251610868292No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT740.16671923579507053No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.15996034785743252No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT690.15545442256567388No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.15545442256567388No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA680.15320145991979453No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT670.1509484972739152No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA660.14869553462803586No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.14869553462803586No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT640.1441896093362772No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT640.1441896093362772No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT630.14193664669039788No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.1351777587527599No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.13292479610688054No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC590.13292479610688054No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC570.12841887081512188No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.12841887081512188No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC560.12616590816924256No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT560.12616590816924256No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.12391294552336324No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.12391294552336324No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC550.12391294552336324No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC550.12391294552336324No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT540.12165998287748389No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA530.11940702023160456No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT530.11940702023160456No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA530.11940702023160456No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA520.11715405758572522No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA510.11490109493984589No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC500.11264813229396656No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT500.11264813229396656No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT490.11039516964808722No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT490.11039516964808722No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT490.11039516964808722No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT480.10814220700220792No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCT480.10814220700220792No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA480.10814220700220792No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC480.10814220700220792No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.10588924435632859No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.10363628171044925No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.10363628171044925No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.1013833190645699No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.1013833190645699No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA450.1013833190645699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)