FastQCFastQC Report
Sun 21 May 2023
EGAF00007838229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54234
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA23864.3994542169119No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT22124.0786222664749054No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21383.942176494449976No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT17533.232289707563521No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT15532.8635173507393885No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14852.7381347494191837No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14462.666224139838478No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12412.288232474093742No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9471.7461371095622673No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9151.687133532470406No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9131.6834458089021647No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8701.6041597521849762No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG7981.4714017037282885No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG7671.414241988420548No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6981.2870155253162223No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6671.2298558100084818No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6621.2206365010878784No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT6381.1763838182689825No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6241.1505697532912933No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6161.135818859018328No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6061.1173802411771214No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6011.108160932256518No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT5941.0952538997676735No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5851.0786591437105875No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5801.0694398347899843No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5501.0141239812663643No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5501.0141239812663643No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5370.9901537780727956No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5340.9846221927204336No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5290.9754028837998304No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4680.86292731496847No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4520.8334255264225394No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4370.8057675996607293No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4350.8020798760924881No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.7191060958070583No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3830.7061990633182137No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3610.6656341040675591No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3510.6471954862263525No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3500.6453516244422318No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3460.6379761773057492No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3280.6047866651915772No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3210.5918796327027326No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2940.5420953645314747No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.5255006084743887No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2730.5033742670649408No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.4812479256554929No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.4683408931666483No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2500.46096544603016554No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2430.4480584135413209No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1990.3669284950400118No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1950.35955304790352915No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1940.3577091861194085No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.35217760076704646No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1910.35217760076704646No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.35033373898292586No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.35033373898292586No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1840.33927056827820185No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1820.3355828447099606No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1790.33005125935759855No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.3282073975734779No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.32451967400523657No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1740.32083195043699525No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1740.32083195043699525No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1690.3116126415163919No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1650.3042371943799093No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.29501788545930596No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1580.29133016189106464No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.29133016189106464No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.289486300106944No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1570.289486300106944No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1520.2802669911863407No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1500.27657926761809937No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.2655160969133754No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.2655160969133754No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.26182837334513404No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1360.2507652026404101No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1340.24707747907216876No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1330.2452336172880481No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1320.2433897555039274No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.23232658479920343No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1250.23048272301508277No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1240.22863886123096214No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.22495113766272082No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.22126341409447947No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1180.21757569052623815No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.2157318287421175No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1170.2157318287421175No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.2138879669579968No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.2065125198215142No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1110.20466865803739354No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.2009809344691522No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1070.19729321090091087No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1060.1954493491167902No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1050.19360548733266955No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1030.1899177637644282No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1020.18807390198030757No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1000.18438617841206623No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT990.18254231662794557No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT990.18254231662794557No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC940.17332300770734227No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA890.16410369878673894No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA890.16410369878673894No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA880.16225983700261828No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC870.16041597521849763No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA850.15672825165025628No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.153040528082015No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.14750894272965298No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA800.14750894272965298No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT790.14566508094553232No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.14382121916141166No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA780.14382121916141166No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.141977357377291No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.14013349559317034No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.136445772024929No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA730.13460191024080834No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.13460191024080834No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.13460191024080834No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.1327580484566877No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT700.12907032488844636No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT700.12907032488844636No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT680.12538260132020504No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT680.12538260132020504No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.11985101596784305No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA640.1180071541837224No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC630.11616329239960171No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.11247556883136041No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT610.11247556883136041No Hit
ATCCTACATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.11063170704723974No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.10878784526311908No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA590.10878784526311908No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA590.10878784526311908No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.10878784526311908No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA560.1032562599107571No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC560.1032562599107571No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA550.10141239812663642No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG550.10141239812663642No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT550.10141239812663642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)