FastQCFastQC Report
Sun 21 May 2023
EGAF00007838237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49716
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT23884.80328264542602No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT15823.1820741813500684No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15543.125754284335023No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15323.0815029366803444No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11702.3533671252715425No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG10442.0999275887038378No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10432.0979161638104435No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9721.9551049963794351No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8951.80022527958806No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8401.6895969104513637No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7771.5628771421675114No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7761.560865717274117No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7361.480408721538338No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT7281.4643173223911818No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7201.448225923244026No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6541.3154718802799903No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6181.243060584117789No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5941.1947863866763215No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5661.1384664896612762No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5411.088180867326414No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5001.0057124466972405No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4940.9936438973368735No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4650.9353125754284335No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4550.9151983264944886No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4540.9131869016010942No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4410.8870383779869659No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4270.8588784294794433No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4270.8588784294794433No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4100.824684206291737No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3960.7965242577842143No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3830.7703757341700861No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3720.7482500603427468No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3390.6818730388607289No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3280.6597473650333896No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3070.6175074422721055No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2840.5712446697240325No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.5652103950438491No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2720.5471075710032987No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2660.5350390216429319No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2510.5048676482420147No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2490.5008447984552257No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2450.49279909888164775No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2450.49279909888164775No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2280.4586048756939416No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2150.43245635207981337No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.4284335022930244No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2120.42642207739962995No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2100.42239922761284093No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2000.4022849786788961No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1990.40027355378550167No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1950.3922278542119237No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1930.3882050044251348No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1920.3861935795317403No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.38015930485155686No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1850.37211360527797893No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1810.364067905704401No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1800.3620564808110065No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.35401078123742863No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1740.3499879314506396No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1730.34797650655724516No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1720.34596508166385065No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1710.3439536567704562No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1660.33389653230348376No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1650.3318851074100893No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1630.32786225762330035No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1550.3117708584761445No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1520.305736583795961No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1470.29567945932898865No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.29567945932898865No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.28562233486201627No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1400.2815994850752273No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1390.2795880601818328No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1370.27556521039504384No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1360.2735537855016493No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.26349666103467695No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1310.26349666103467695No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1270.255450961461099No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.25343953656770457No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.2514281116743101No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1250.2514281116743101No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.2514281116743101No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.2474052618875211No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1210.24338241210073217No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.2393595623139432No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1180.23734813742054872No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1110.22326816316678735No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1100.22125673827339287No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1100.22125673827339287No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1100.22125673827339287No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1070.21522246359320943No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1070.21522246359320943No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1030.20717676401963153No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1020.20516533912623702No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.20315391423284251No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA990.19913106444605358No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA990.19913106444605358No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC960.19309678976587016No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.19108536487247565No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA900.18102824040550325No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.18102824040550325No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT900.18102824040550325No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.18102824040550325No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT880.17700539061871431No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC870.1749939657253198No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA850.17097111593853087No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT840.1689596910451364No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC830.16694826615174188No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT830.16694826615174188No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA830.16694826615174188No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.1649368412583474No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT820.1649368412583474No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT810.16292541636495295No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.15890256657816396No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA780.1568911416847695No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.14884544211119158No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT740.14884544211119158No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA720.1448225923244026No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.14281116743100813No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT700.14079974253761365No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA670.1347654678574302No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT670.1347654678574302No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.13275404296403573No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA660.13275404296403573No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.13074261807064125No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCTAGCT650.13074261807064125No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC650.13074261807064125No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.12873119317724674No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA620.1247083433904578No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.1247083433904578No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT610.12269691849706332No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.12269691849706332No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.12269691849706332No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC610.12269691849706332No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA580.11666264381687988No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA580.11666264381687988No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACATATGGGGCGAGCGTTA570.1146512189234854No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT570.1146512189234854No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACACT540.10861694424330195No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA540.10861694424330195No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT540.10861694424330195No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC540.10861694424330195No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10660551934990749No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT520.10459409445651299No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT510.10258266956311851No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC510.10258266956311851No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.10057124466972403No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.10057124466972403No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG500.10057124466972403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)