FastQCFastQC Report
Sun 21 May 2023
EGAF00007838280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41926
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11292.692839765300768No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10672.5449601679148977No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8291.9772933263368793No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7761.8508801221199256No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7731.8437246577302866No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7581.807947335782092No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7351.7530887754615274No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7281.7363926918857033No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6141.4644850450794258No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5911.4096264847588609No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5891.4048561751657684No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5881.402471020369222No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5691.3571530792348423No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5561.3261460668797405No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5101.2164289462386109No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5021.1973477078662405No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4951.1806516242904166No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4861.1591852311214998No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4731.1281782187663978No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4631.104326670800935No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4581.0924008968182035No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4431.056623574870009No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4231.008920478939083No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4211.0041501693459904No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4120.9826837761770738No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4030.9612173830081573No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3940.9397509898392405No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.9373658350426942No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.9302103706530553No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3890.927825215856509No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3800.9063588226875924No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3780.9015885130944999No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3710.8848924295186757No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3560.8491151075704813No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3550.8467299527739349No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3240.7727901540809999No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3230.7704049992844536No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3010.717931593760435No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2840.677383962219148No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2750.6559175690502314No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2730.6511472594571388No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.6463769498640461No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2700.6439917950674998No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.6177550923054906No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2520.6010590087296666No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2510.5986738539331202No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2480.5915183895434815No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2370.5652816867814721No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2330.555741067595287No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2290.5462004484091018No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2170.5175785908505462No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1970.4698754949196203No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.4627200305299814No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1940.4627200305299814No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.4531794113437962No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.45079425654724997No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1880.44840910175070364No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.44602394695415737No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1870.44602394695415737No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.44125363736106477No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1730.4126317798025092No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1700.4054763154128703No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1670.3983208510232314No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1650.3935505414301388No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1600.38162476744740736No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1540.36731383866812956No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1520.36254352907503695No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1480.35300290988885175No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1400.33392167151648144No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.33392167151648144No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1390.3315365167199351No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.32915136192338884No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1340.31961074273720363No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.3172255879406573No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1320.3148404331441111No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.3148404331441111No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA1300.3100701235510185No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1290.30768496875447215No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.30768496875447215No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.3005295043648333No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1200.2862185755855555No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1200.2862185755855555No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.28144826599246286No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.27906311119591665No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1160.2766779563993703No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.2719076468062777No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1130.26952249200973144No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.25759671802699996No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1070.2552115632304537No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1060.25282640843390736No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.2504412536373611No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT980.233745170061537No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA970.2313600152649907No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT930.22181939607880552No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC930.22181939607880552No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA900.21466393168916661No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.21466393168916661No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT860.20512331250298146No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC860.20512331250298146No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG850.20273815770643516No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC850.20273815770643516No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC830.19796784811334256No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA810.19319753852024998No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA790.18842722892715738No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCTGGCGTTA780.18604207413061108No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA770.18365691933406478No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT760.18127176453751848No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT760.18127176453751848No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA750.17888660974097217No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT750.17888660974097217No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA750.17888660974097217No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.17888660974097217No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA740.17650145494442587No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT730.1741163001478796No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.1741163001478796No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.1741163001478796No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT720.1717311453513333No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC720.1717311453513333No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC700.16696083575824072No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCTGGCGTTA700.16696083575824072No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA690.16457568096169442No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT680.16219052616514812No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.16219052616514812No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC670.15980537136860182No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA660.15742021657205554No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA660.15742021657205554No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC650.15503506177550924No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT640.15264990697896294No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA630.15026475218241664No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.14787959738587036No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA610.14549444258932404No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACT600.14310928779277776No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.14310928779277776No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCTGGCGTTA590.14072413299623143No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACT590.14072413299623143No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.13833897819968516No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT580.13833897819968516No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT570.13595382340313886No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.13595382340313886No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.13356866860659256No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT550.13118351381004625No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC540.12879835901349998No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA520.12402804942040738No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC500.1192577398273148No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.1192577398273148No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA490.1168725850307685No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA490.1168725850307685No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA490.1168725850307685No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC490.1168725850307685No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.1168725850307685No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC490.1168725850307685No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA490.1168725850307685No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.1144874302342222No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCTGGCGTTA480.1144874302342222No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC470.11210227543767591No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA470.11210227543767591No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.10971712064112961No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA460.10971712064112961No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT450.10733196584458331No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG450.10733196584458331No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC450.10733196584458331No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.10733196584458331No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT430.10256165625149073No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA430.10256165625149073No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG430.10256165625149073No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA430.10256165625149073No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA420.10017650145494443No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT420.10017650145494443No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT420.10017650145494443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)