FastQCFastQC Report
Sun 21 May 2023
EGAF00007838287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48106
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21244.4152496570074415No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18583.8623040784933274No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA12942.6898931526212944No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11802.4529164761152455No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA11552.4009479067060244No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11292.346900594520434No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9211.9145220970357129No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7291.5154034839728932No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7071.4696711428927784No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6921.4384900012472457No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6771.407308859601713No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6651.3823639462852866No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6581.3678127468507046No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6431.3366316052051719No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6381.3262378913233277No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6021.251403151374049No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5631.1703321830956637No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5411.124599842015549No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5411.124599842015549No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5061.051843844842639No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4680.9728516193406228No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA4420.9188043071550326No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4050.8418908244293852No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC3940.8190246538893278No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3770.7836860266910572No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3750.7795285411383196No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3590.7462686567164178No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3510.7296387145054671No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3430.7130087722945163No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3410.7088512867417786No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3410.7088512867417786No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3360.6984575728599343No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3320.6901426017544589No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.6589614601089261No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3020.6277803184633933No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.6111503762524425No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2900.6028354051469671No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2870.5965991768178606No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.5882842057123852No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2770.575811749054172No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2730.5674967779486967No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.5529455785141146No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2610.5425518646322705No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2600.5404731218559016No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2580.5363156363031638No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2540.5280006651976884No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2480.5155282085394753No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2310.4801895813412048No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.4801895813412048No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC2180.45316592524840976No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC2160.449008439695672No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2160.449008439695672No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC2150.44692969691930323No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC2130.4427722113665655No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2130.4427722113665655No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.39496112751008194No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1870.38872489918097536No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.38872489918097536No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC1830.38040992807549995No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1760.365858728640918No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1740.36170124308818025No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1740.36170124308818025No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1720.35754375753544254No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.35754375753544254No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1700.3533862719827049No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1620.336756329771754No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1570.3263626158899098No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1550.3222051303371721No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1550.3222051303371721No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1530.31804764478443437No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1520.31596890200806554No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.31389015923169666No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1510.31389015923169666No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.30973267367895896No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.30557518812622125No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1460.30349644534985243No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1460.30349644534985243No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1410.29310273146800814No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC1410.29310273146800814No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1400.2910239886916393No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.28894524591527043No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1380.2868665031389016No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1380.2868665031389016No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1360.2827090175861639No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1350.280630274809795No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1340.2785515320334262No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1310.2723153037043196No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1280.2660790753752131No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1260.2619215898224754No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2619215898224754No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2619215898224754No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1250.25984284704610655No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1240.25776410426973767No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.25360661871699997No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.24944913316426223No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1180.24529164761152455No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1140.23697667650604912No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC1140.23697667650604912No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1110.2307404481769426No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1080.224504219847836No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1050.21826799151872947No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.21618924874236062No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT990.20579553486051635No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.19955930653140982No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT960.19955930653140982No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT940.1954018209786721No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA940.1954018209786721No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG900.18708684987319668No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.18500810709682783No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC890.18500810709682783No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA860.17877187876772127No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC840.17461439321498357No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC840.17461439321498357No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA830.1725356504386147No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.1725356504386147No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT810.168378164885877No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC810.168378164885877No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT810.168378164885877No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA800.16629942210950818No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT800.16629942210950818No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT800.16629942210950818No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA780.16214193655677045No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC760.15798445100403277No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA760.15798445100403277No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT750.1559057082276639No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT750.1559057082276639No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA750.1559057082276639No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG740.15382696545129507No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA740.15382696545129507No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.15174822267492621No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT720.14966947989855733No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT720.14966947989855733No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT700.14551199434581966No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC690.1434332515694508No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACC690.1434332515694508No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT670.1392757660167131No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT670.1392757660167131No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC650.1351182804639754No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.1351182804639754No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC650.1351182804639754No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG630.1309607949112377No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA630.1309607949112377No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.1309607949112377No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT630.1309607949112377No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT610.12680330935849998No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT600.12472456658213112No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT600.12472456658213112No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC580.12056708102939342No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.12056708102939342No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.11848833825302456No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG570.11848833825302456No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.11848833825302456No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA560.11640959547665572No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.11640959547665572No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC550.11433085270028687No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACC550.11433085270028687No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT540.112252109923918No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT530.11017336714754916No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.11017336714754916No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC520.10809462437118031No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.10601588159481144No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.10601588159481144No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG510.10601588159481144No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.10601588159481144No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA500.1039371388184426No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.10185839604207375No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA490.10185839604207375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)