FastQCFastQC Report
Sun 21 May 2023
EGAF00007838292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48523
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15103.1119263029903346No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13802.8440121179646765No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13462.7739422541887353No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13372.755394349071574No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12182.5101498258557795No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10662.196896317210395No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10492.1618613853224242No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8011.6507635554273232No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7941.6363374070028647No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7741.5951198400758402No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6811.4034581538651774No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6791.3993363971724748No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6761.3931537621334211No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6451.3292665333965337No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6291.2962924798549142No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6271.2921707231622117No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG6221.2818663314304557No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6151.2674401830059971No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6141.265379304659646No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6131.2633184263132946No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6081.2530140345815388No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6061.2488922778888363No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5991.2344661294643777No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5971.2303443727716752No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5861.207674710961812No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5361.1046307936442512No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5241.0799002534880366No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5131.0572305916781732No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4850.9995259979803393No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4610.95006491766791No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4210.8676297838138616No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4140.853203635389403No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4080.8408383653112956No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.8325948519258908No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.8325948519258908No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3820.7872555283061642No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3700.7625249881499495No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3680.7584032314572471No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3560.7336726913010325No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3540.7295509346083301No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3360.6924551243740082No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3300.6800898542959009No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.6532984357933351No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3160.6512375574469839No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3150.6491766791006327No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3150.6491766791006327No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3020.6223852605980669No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2870.5914720854027986No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2730.5626197885538817No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.5502545184757744No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2620.5399501267440183No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2480.5110978298951013No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2470.5090369515487501No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2300.47400201966077943No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2220.4575149928899697No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2150.4430888444655112No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2090.43072357438740394No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2040.4204191826556478No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.4162974259629454No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.4059930342311893No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.3956886424994333No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.3895060074603796No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1880.3874451291140284No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1720.354471075572409No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1680.3462275621870041No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1620.3338622921088968No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1610.33180141376254557No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1580.32561877872349193No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.3235579003771407No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.32149702203078956No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1530.31531438699173586No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1510.31119263029903343No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.30500999525997985No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1420.2926447251818725No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1390.28646209014281887No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.28234033345011644No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.26585330667930673No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1270.2617315499866043No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.2576097932939018No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.25348803660119945No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1180.24318364486944338No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1180.24318364486944338No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.23700100983038971No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1100.22669661809863362No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.21639222636687755No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.21639222636687755No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1050.21639222636687755No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1040.21433134802052636No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1030.21227046967417515No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1000.2060878346351215No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.20402695628877027No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT980.20196607794241905No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT970.19990519959606784No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT960.19784432124971665No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT950.19578344290336538No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT920.18960080786431177No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.18753992951796056No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.18547905117160934No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC900.18547905117160934No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA880.18135729447890692No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT870.1792964161325557No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA840.17311378109350206No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.1669311460544484No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.16074851101539478No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC780.16074851101539478No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT770.15868763266904354No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA770.15868763266904354No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.15868763266904354No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC760.15662675432269235No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.15662675432269235No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC740.15250499762998992No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.15250499762998992No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC730.15044411928363868No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.15044411928363868No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC710.14632236259093626No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC710.14632236259093626No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.14426148424458504No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA700.14426148424458504No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT690.14220060589823383No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC680.14013972755188261No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC670.1380788492055314No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.13395709251282897No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.13395709251282897No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT640.13189621416647776No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT640.13189621416647776No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA630.12983533582012655No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT620.12777445747377533No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.12777445747377533No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.12777445747377533No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12365270078107289No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12365270078107289No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC590.12159182243472169No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT580.11953094408837046No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT580.11953094408837046No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.11540918739566804No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC560.11540918739566804No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC540.11128743070296561No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10716567401026318No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA510.10510479566391195No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA510.10510479566391195No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.10510479566391195No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA510.10510479566391195No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT510.10510479566391195No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.10098303897120953No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC490.10098303897120953No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.10098303897120953No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.10098303897120953No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT490.10098303897120953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)