FastQCFastQC Report
Sun 21 May 2023
EGAF00007838314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46632
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23545.048035683650712No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23174.968691027620518No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9722.0844055584148222No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9111.9535940984731512No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9021.9342940470063477No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8951.9192828958655002No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8211.7605935838051125No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8031.7219934808715045No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7681.6469377251672672No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7661.6426488248413105No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7421.5911820209298335No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7301.565448618974095No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6161.320981300394579No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6031.293103448275862No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5591.1987476411048208No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5541.1880253902899298No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5411.160147538171213No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5151.1043918339337795No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4941.059358380511237No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4891.0486361296963458No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4741.0164693772516726No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4620.9907359752959342No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4580.9821581746440213No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4510.9671470235031738No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4510.9671470235031738No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3600.7720020586721564No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.7591353576942872No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3520.7548464573683308No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3470.7441242065534397No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.7098130039457884No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3210.6883685023160062No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3200.686224052153028No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3200.686224052153028No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA3140.6733573511751587No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3120.6690684508492023No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.6647795505232458No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3030.6497683993823984No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.6304683479155945No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2890.6197460971007034No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.6025904957968776No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.6004460456338995No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2780.5961571453079431No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2700.5790015440041174No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2640.5661348430262481No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2640.5661348430262481No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2630.5639903928632698No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2540.544690341396466No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2440.5232458397666838No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2370.5082346886258363No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2150.4610567850403157No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1960.42031223194372963No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1940.41602333161777316No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1910.40958998112883854No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.4031566306399039No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1820.39028992966203463No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.3860010293360782No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1780.3817121290101218No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.3817121290101218No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1770.3795676788471436No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.3709898781952307No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1700.3645565277062961No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1680.3602676273803397No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1680.3602676273803397No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1650.353834276891405No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1650.353834276891405No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1650.353834276891405No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1620.3474009264024704No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1580.33882312575055756No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1550.3323897752616229No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1470.31523417395779724No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1470.31523417395779724No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1430.30665637330588436No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1310.2809229713501458No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.2766340710241894No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1280.27448962086121115No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1270.272345170698233No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1270.272345170698233No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.2616229198833419No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.2616229198833419No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1200.2573340195573855No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1180.2530451192314291No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.24875621890547264No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1150.2466117687424944No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1140.24446731857951623No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1090.23374506776462517No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.23374506776462517No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.23160061760164694No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.23160061760164694No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1070.22945616743866873No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.22516726711271232No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.21658946646079943No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.21444501629782126No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA980.21015611597186482No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT890.1908560645050609No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT880.1887116143420827No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA880.1887116143420827No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT870.18656716417910446No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.18227826385314805No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT840.18013381369016984No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA840.18013381369016984No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT830.17798936352719164No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA830.17798936352719164No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA820.1758449133642134No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC820.1758449133642134No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT810.1737004632012352No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.16941156287527878No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC780.16726711271230058No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC780.16726711271230058No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC770.16512266254932237No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.16083376222336593No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA740.15868931206038772No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA740.15868931206038772No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT740.15868931206038772No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT730.1565448618974095No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.1544004117344313No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC720.1544004117344313No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.15225596157145307No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT710.15225596157145307No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC710.15225596157145307No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.15225596157145307No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC710.15225596157145307No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.15011151140847487No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.14796706124549666No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA690.14796706124549666No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.14796706124549666No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA690.14796706124549666No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.14796706124549666No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA680.14582261108251846No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC680.14582261108251846No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA680.14582261108251846No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.14367816091954022No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.14153371075656201No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC630.1351003602676274No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT620.13295591010464916No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC610.13081145994167095No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC610.13081145994167095No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA600.12866700977869275No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA600.12866700977869275No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12866700977869275No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT590.12652255961571454No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC580.12437810945273632No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC550.11794475896380167No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.11794475896380167No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT540.11580030880082347No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT530.11365585863784525No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.11365585863784525No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10936695831188882No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.10936695831188882No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT510.10936695831188882No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10722250814891063No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.10722250814891063No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT500.10722250814891063No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA490.10507805798593241No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT490.10507805798593241No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC480.1029336078229542No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.1029336078229542No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.1029336078229542No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.10078915765997598No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG470.10078915765997598No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC470.10078915765997598No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT470.10078915765997598No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT470.10078915765997598No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.10078915765997598No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA470.10078915765997598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)