FastQCFastQC Report
Sun 21 May 2023
EGAF00007838315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46632
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22624.850746268656716No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20764.451878538342769No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11152.391061931720707No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9632.0651055069480186No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8541.8313604391833933No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8191.756304683479156No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7981.7112712300566133No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7971.7091267798936352No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7271.5590152684851604No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7041.5096929147366616No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6571.4089037570766856No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6501.393892605935838No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6461.385314805283925No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6081.303825699090753No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5871.2587922456682106No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5651.2116143420826901No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5221.1194029850746268No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5211.1172585349116486No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5171.1086807342597358No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4981.0679361811631498No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4741.0164693772516726No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4290.9199691199176531No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4280.9178246697546749No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4150.8899468176359582No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4070.8727912163321324No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3980.8534911648653285No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3920.8406244638874593No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3750.8041688111168297No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3650.7827243094870475No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3640.7805798593240693No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3620.776290958998113No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3400.7291130554125922No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3210.6883685023160062No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3170.6797907016640933No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3120.6690684508492023No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2960.6347572482415509No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2920.626179447589638No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2830.6068793961228341No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2790.5983015954709212No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2720.5832904443300738No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2660.5704237433522045No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2570.5511236918854006No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2530.5425458912334877No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2510.5382569909075313No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2450.525390289929662No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2410.5168124892777493No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2370.5082346886258363No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2350.5039457882998799No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.48250128667009773No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2160.46320123520329387No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2090.44819008406244637No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.44175673357351175No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1890.40530108080288213No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1870.4010121804769258No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1850.3967232801509693No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1800.3860010293360782No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1790.3838565791731No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1790.3838565791731No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.3795676788471436No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1760.3774232286841654No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.3752787785211872No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1730.3709898781952307No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1690.3624120775433179No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1680.3602676273803397No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1630.34954537656544865No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1570.33667867558757936No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1560.33453422542460115No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1550.3323897752616229No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1520.32595642477268827No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1470.31523417395779724No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.3109452736318408No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1440.3088008234688626No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1400.30022302281694974No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1290.2766340710241894No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.27448962086121115No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1280.27448962086121115No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.27448962086121115No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.272345170698233No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1240.2659118202092983No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.2637673700463201No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1200.2573340195573855No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.2573340195573855No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1200.2573340195573855No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.2573340195573855No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1200.2573340195573855No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.25518956939440723No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1130.242322868416538No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1080.23160061760164694No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1070.22945616743866873No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1050.22516726711271232No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1030.22087836678675588No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.21444501629782126No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA970.20801166580888658No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT950.2037227654829302No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT930.19943386515697376No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.19943386515697376No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.19514496483101731No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC900.1930005146680391No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT890.1908560645050609No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC880.1887116143420827No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA880.1887116143420827No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC880.1887116143420827No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT880.1887116143420827No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.1887116143420827No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.18656716417910446No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC870.18656716417910446No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.18442271401612628No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT850.18227826385314805No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC850.18227826385314805No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA840.18013381369016984No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.18013381369016984No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC830.17798936352719164No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC810.1737004632012352No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC810.1737004632012352No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC800.171556013038257No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT760.16297821238634413No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.15868931206038772No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT740.15868931206038772No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC740.15868931206038772No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.15868931206038772No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA740.15868931206038772No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT730.1565448618974095No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA720.1544004117344313No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.1544004117344313No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA720.1544004117344313No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA710.15225596157145307No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT710.15225596157145307No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC710.15225596157145307No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC690.14796706124549666No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT690.14796706124549666No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA690.14796706124549666No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.14796706124549666No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC680.14582261108251846No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA660.14153371075656201No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.13724481043060557No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.1351003602676274No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT630.1351003602676274No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA630.1351003602676274No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.1351003602676274No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT620.13295591010464916No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA610.13081145994167095No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.13081145994167095No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT610.13081145994167095No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.12652255961571454No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT580.12437810945273632No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT580.12437810945273632No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT580.12437810945273632No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT580.12437810945273632No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.12223365928975811No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.12223365928975811No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.1200892091267799No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC560.1200892091267799No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA540.11580030880082347No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.11580030880082347No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCT540.11580030880082347No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.11365585863784525No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.11151140847486705No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.11151140847486705No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.10936695831188882No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.10936695831188882No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTG500.10722250814891063No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10722250814891063No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.10507805798593241No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT470.10078915765997598No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT470.10078915765997598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)