FastQCFastQC Report
Sun 21 May 2023
EGAF00007838325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40303
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14653.634965139071533No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14173.515867305163387No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13203.2751904324740093No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13003.2255663350122816No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11992.9749646428305585No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8612.136317395727365No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8242.0445128154231695No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7871.9527082351189737No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7541.8708284743071235No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7121.766617869637496No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6081.5085725628365134No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5961.478798104359477No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5751.4266928020246632No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5691.411805572786145No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5381.3348882217204674No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5221.2951889437510855No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5201.2902265340049128No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5021.245564846289358No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5021.245564846289358No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5011.2430836414162716No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4871.2083467731930626No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4851.20338436344689No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4821.1959407488276306No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4621.1463166513659033No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4451.104136168523435No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4251.0545120710617075No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4141.0272188174577574No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3920.9726323102498574No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3720.92300821278813No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3610.8957149591841799No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3410.8460908617224525No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3270.8113539934992432No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3250.8063915837530704No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3150.7815795350222067No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3100.769173510656775No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.7642111009106022No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3080.7642111009106022No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2990.7418802570528248No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2970.7369178473066521No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2780.6897749547180111No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.66992531573332No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.6600004962409746No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2350.5830831451752971No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2240.5557898915713471No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2240.5557898915713471No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2190.5433838672059152No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.5309778428404833No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.4863161551249287No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.47391013075949684No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.47142892588641044No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1810.44909808202863316No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1800.44661687715554677No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.4267672381708557No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.41436121380542396No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1660.41188000893233756No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.41188000893233756No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.40939880405925116No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.3870679602014738No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1550.3845867553283875No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1530.37962434558221475No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1410.3498498871051783No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.33992506761283275No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1350.33496265786666No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1300.3225566335012282No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1280.31759422375505547No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1270.3151130188819691No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1260.31263181400888274No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1180.29278217502419174No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.28285735553184627No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1100.2729325360395008No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1090.2704513311664144No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1080.267970126293328No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1050.26052651167406893No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1040.25804530680098253No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.25804530680098253No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1040.25804530680098253No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.25804530680098253No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1030.2555641019278962No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1000.2481204873086371No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1000.2481204873086371No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT970.24067687268937796No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.23571446294320522No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC920.22827084832394612No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT910.22578964345085972No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.22578964345085972No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA900.22330843857777338No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT900.22330843857777338No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA880.21834602883160065No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA870.21586482395851425No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA810.20097759471999604No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC780.1935339801007369No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG760.18857157035456418No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC750.1860903654814778No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC740.18360916060839144No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC730.18112795573530507No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT720.1786467508622187No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT710.1761655459891323No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA690.17120313624295957No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC680.1687219313698732No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA680.1687219313698732No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA680.1687219313698732No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT660.16375952162370047No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.1612783167506141No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT650.1612783167506141No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC640.15879711187752774No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC630.15631590700444137No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA620.153834702131355No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.153834702131355No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC610.15135349725826863No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA610.15135349725826863No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC610.15135349725826863No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA600.14887229238518224No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA580.1439098826390095No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.1439098826390095No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA570.14142867776592313No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT560.13894747289283677No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA560.13894747289283677No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.1364662680197504No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA540.133985063146664No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG530.13150385827357763No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC530.13150385827357763No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA530.13150385827357763No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT520.12902265340049127No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC520.12902265340049127No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC520.12902265340049127No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.1265414485274049No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.12406024365431854No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA500.12406024365431854No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT490.12157903878123218No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.12157903878123218No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC480.1190978339081458No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT480.1190978339081458No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.1190978339081458No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.11661662903505943No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC450.11165421928888669No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT450.11165421928888669No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA450.11165421928888669No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT450.11165421928888669No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.10917301441580032No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA440.10917301441580032No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT420.10421060466962756No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC420.10421060466962756No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA410.10172939979654119No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.10172939979654119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)