FastQCFastQC Report
Sun 21 May 2023
EGAF00007838331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50746
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA32716.445828242620108No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA29845.880266424939896No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT22704.473258976076932No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA22414.416111614708548No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA17783.503724431482284No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12532.4691601308477513No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11352.236629488038466No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10642.09671698261932No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10432.055334410593938No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9721.9154219051747923No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9551.8819217278209122No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9341.8405391557955306No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8671.7085090450478857No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8591.692744255704883No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8581.6907736570370078No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8561.6868324597012572No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT7401.4582430142277223No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6801.3400070941552042No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6291.239506562093564No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5671.1173294446852955No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5621.107476451345919No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5611.1055058526780437No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5251.0345643006345326No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4930.9715051432625231No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4290.8453868285185039No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4050.7980924604894968No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3960.7803570724786191No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT3530.695621329759981No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3430.6759153430812281No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3430.6759153430812281No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3340.6581799550703504No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3320.6542387577345997No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3130.6167973830449691No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3040.5990619950340914No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3020.5951207976983407No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3000.5911796003625901No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2840.5596500216765854No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.5576794230087101No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2710.5340322389942065No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.49264966696882506No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2480.48870846963307457No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2390.4709730816221968No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2290.4512670949434438No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.43944350293619205No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.4355023056004414No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2070.4079139242501872No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.40397272691443664No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1940.3822961415678083No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.3763843455641824No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1900.3744137468963071No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1830.36061955622118No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1810.35667835888542937No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.35273716154967877No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1780.3507665628818035No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1730.340913569542427No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.3093839908564222No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1500.29558980018129505No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1500.29558980018129505No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2916486028455445No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1470.2896780041776692No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1450.28573680684191854No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.26603082016316554No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1310.25814842549166434No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1200.23647184014503608No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGGAGGGTGCGAGCGTTA1150.22661884680565958No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGGAGGGTGCGAGCGTTA1150.22661884680565958No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1100.21676585346628308No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1090.21479525479840778No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.20888345879478185No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.20494226145903127No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1010.19903046545540534No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.19903046545540534No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.18917747211602887No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA960.18917747211602887No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.18523627478027826No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT930.18326567611240294No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT920.18129507744452764No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA910.17932447877665236No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC890.17538328144090176No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT890.17538328144090176No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAACACGGAGGGTGCGAGCGTTA890.17538328144090176No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAACACGGAGGGTGCGAGCGTTA880.17341268277302643No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.16750088676940053No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG830.16355968943364993No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT820.16158909076577466No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT760.14976549875852285No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC740.14582430142277225No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT710.13991250541914635No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT710.13991250541914635No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA710.13991250541914635No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA690.13597130808339575No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA690.13597130808339575No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT670.13203011074764515No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT650.12808891341189452No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.12808891341189452No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.12611831474401924No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.12611831474401924No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.12611831474401924No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC630.12414771607614393No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.12217711740826864No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA620.12217711740826864No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG610.12020651874039333No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.12020651874039333No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT610.12020651874039333No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT570.11232412406889214No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.11232412406889214No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT570.11232412406889214No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.11232412406889214No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA560.11035352540101682No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.11035352540101682No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.11035352540101682No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC550.10838292673314154No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.10838292673314154No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.10641232806526622No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA530.10444172939739092No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA530.10444172939739092No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10247113072951564No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG510.10050053206164032No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC510.10050053206164032No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA510.10050053206164032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)