FastQCFastQC Report
Sun 21 May 2023
EGAF00007838344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56564
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13792.4379463970016264No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13552.3955165829856444No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13302.3513188600523303No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12922.284138321193692No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12762.2558517785163708No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9811.7343186479032602No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9421.6653702001272894No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9381.6582985644579593No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9141.615868750441977No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9011.5928859345166537No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8631.5257053956580158No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8511.5044904886500248No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8401.4850434905593664No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7541.3330033236687644No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7391.3064846899087759No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6991.2357683332154727No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6841.209249699455484No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6291.1120147090021923No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6221.0996393465808643No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6221.0996393465808643No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6111.0801923484902058No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5811.0271550809702283No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5731.013011809631568No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5721.011243900714235No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5540.9794215402022488No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5450.9635103599462556No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5180.915776819178276No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4980.8804186408316244No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4760.8415246446503075No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4660.8238455554769819No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4580.8097022841383212No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4490.793791103882328No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4290.7584329255356764No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3960.7000919312637013No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3930.6947882045117035No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3930.6947882045117035No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3910.6912523866770384No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3810.6735732975037126No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3800.6718053885863801No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3670.6488225726610566No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3650.6452867548263913No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3500.6187681210664027No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3290.5816420338024185No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3280.5798741248850858No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.5781062159677534No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2960.5233010395304434No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.491478679018457No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2630.4649600452584683No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2590.457888409589138No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2540.4490488650024751No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2510.4437451382504774No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2500.44197722933314476No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2500.44197722933314476No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2420.4278339579944841No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2420.4278339579944841No Hit
ATGTATCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2410.42606604907715157No Hit
ATGTATCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2400.4242981401598189No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2370.41899441340782123No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.40308323315182804No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2270.4013153242344954No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2250.39777950639983023No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2070.36595714588784384No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2040.36065341913584614No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2040.36065341913584614No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1990.3518138745491832No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1950.34474223887985295No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.3412064210451877No Hit
ATGTATCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1900.33590269429319003No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1770.3129198783678665No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1740.30761615161586875No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1660.29347288027720814No Hit
ATGTATCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1630.2881691535252104No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1610.2846333356905452No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1560.2757937911038823No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1550.2740258821865497No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1550.2740258821865497No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1550.2740258821865497No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1520.268722155434552No Hit
ATGTATCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2616505197652217No Hit
ATGTATCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1450.256346793013224No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1410.24927515734389366No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1410.24927515734389366No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.2422035216745633No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1350.23866770383989816No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.23866770383989816No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1350.23866770383989816No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1330.235131886005233No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1290.2280602503359027No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.22452443250123755No Hit
ATGTATCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.22275652358390496No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.21038116116257688No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1180.20861325224524435No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1160.20507743441057916No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.20330952549324657No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.20330952549324657No Hit
ATGTATCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1100.19446998090658368No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1090.1927020719892511No Hit
ATGTATCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1080.19093416307191854No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1080.19093416307191854No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1080.19093416307191854No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1030.18209461848525563No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.17325507389859274No Hit
ATGTATCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT970.17148716498126018No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT970.17148716498126018No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.16971925606392757No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT930.16441552931192985No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC900.15911180255993213No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.1573438936425995No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT890.1573438936425995No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA890.1573438936425995No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC890.1573438936425995No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT870.15380807580793437No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.15380807580793437No Hit
ATGTATCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT860.1520401668906018No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC850.1502722579732692No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA840.14850434905593665No Hit
ATGTATCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.14850434905593665No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA830.14673644013860407No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC830.14673644013860407No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA810.1432006223039389No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.1432006223039389No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA800.14143271338660632No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.14143271338660632No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA800.14143271338660632No Hit
ATGTATCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT800.14143271338660632No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA790.13966480446927373No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT780.13789689555194115No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.1361289866346086No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.1361289866346086No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA740.13082525988261084No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.13082525988261084No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA740.13082525988261084No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT740.13082525988261084No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC730.12905735096527826No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT730.12905735096527826No Hit
ATGTATCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC720.12728944204794568No Hit
ATGTATCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT710.1255215331306131No Hit
ATGTATCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT700.12375362421328054No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG690.12198571529594794No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC680.12021780637861539No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA680.12021780637861539No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG670.11844989746128279No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA660.11668198854395022No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.11491407962661764No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC640.11314617070928505No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC630.11137826179195248No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT630.11137826179195248No Hit
ATGTATCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC630.11137826179195248No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.11137826179195248No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA620.1096103528746199No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA620.1096103528746199No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.10784244395728733No Hit
ATGTATCACCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.10784244395728733No Hit
ATGTATCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.10784244395728733No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT600.10607453503995473No Hit
ATGTATCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT600.10607453503995473No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA600.10607453503995473No Hit
ATGTATCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT590.10430662612262218No Hit
ATGTATCACCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.10430662612262218No Hit
ATGTATCACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT590.10430662612262218No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA580.10253871720528958No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.10077080828795701No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA570.10077080828795701No Hit
ATGTATCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC570.10077080828795701No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG570.10077080828795701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)