FastQCFastQC Report
Sun 21 May 2023
EGAF00007838368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34101
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11833.4691064778158998No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11473.363537726166388No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9802.8738160171255975No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9522.791706988064866No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6872.014603677311516No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6671.955954370839565No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6191.8151960353068826No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5371.5747338787718834No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5111.4984897803583472No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5101.4955573150347496No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5061.4838274537403595No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4861.4251781472684086No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4631.357731444825665No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4571.3401366528840797No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4211.2345679012345678No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4111.2052432479985924No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3931.1524588721738365No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3911.1465939415266415No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3801.1143368229670685No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3631.0644849124659101No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.973578487434386No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3070.9002668543444473No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.8621448051376792No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2910.8533474091668867No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2830.8298876865781062No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2780.8152253599601185No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.8152253599601185No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2760.8093604293129233No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2740.8034954986657282No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2740.8034954986657282No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2700.791765637371338No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2620.7683059147825577No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.7653734494589601No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2590.7595085188117651No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2510.7360487962229847No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2410.7067241429870091No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2400.7037916776634117No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2320.6803319550746313No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.6744670244274361No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2250.6598046978094484No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2230.6539397671622533No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2130.6246151139262779No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.604087856661095No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2040.5982229260138999No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2000.5864930647195097No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.5806281340723146No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.5806281340723146No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1770.519046362276766No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.5161138969531684No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1690.49558663968798566No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1690.49558663968798566No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1620.4750593824228029No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1570.4603970558048151No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.4574645904812175No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1550.45453212515762No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.45453212515762No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1550.45453212515762No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.4457347291868274No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1470.4310724025688396No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.4193425412744495No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.40761267998005923No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1360.3988152840092666No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.3929503533620715No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1330.3900178880384739No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.3870854227148764No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1250.36655816544969355No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1230.36069323480249843No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.35189583883170583No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1170.3430984428609132No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1150.33723351221371806No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.32257118559573034No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1090.3196387202721328No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1070.3137737896249377No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.29324653235975484No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.2873816017125597No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT980.2873816017125597No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC970.28444913638896224No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.2785842057417671No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT940.27565174041816953No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT940.27565174041816953No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.26978680977097447No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG910.26685434444737693No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC900.26392187912377935No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA900.26392187912377935No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG890.2609894138001818No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA880.2580569484765842No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.24925955250579163No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT830.2433946218585965No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT790.23166476056420632No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.23166476056420632No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC790.23166476056420632No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA760.2228673645934137No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT760.2228673645934137No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA740.21700243394621857No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT730.214069968622621No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT720.2111375032990235No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC710.20820503797542594No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC710.20820503797542594No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.20527257265182838No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT700.20527257265182838No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.1994076420046333No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA670.19647517668103576No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG640.1876777807102431No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.1876777807102431No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.18474531538664557No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA620.181812850063048No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT620.181812850063048No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.181812850063048No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.181812850063048No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC620.181812850063048No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC610.17888038473945048No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC600.17594791941585292No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.17301545409225536No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT580.17008298876865782No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC550.16128559279786517No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT550.16128559279786517No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG550.16128559279786517No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.16128559279786517No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA540.1583531274742676No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC540.1583531274742676No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT530.15542066215067007No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT530.15542066215067007No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC520.15248819682707251No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT520.15248819682707251No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT520.15248819682707251No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.14955573150347498No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC510.14955573150347498No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.14955573150347498No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC490.14369080085627986No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT490.14369080085627986No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC480.14075833553268233No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.13782587020908477No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT470.13782587020908477No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC460.13489340488548723No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT460.13489340488548723No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA460.13489340488548723No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT450.13196093956188967No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.13196093956188967No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA450.13196093956188967No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.13196093956188967No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT440.1290284742382921No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT430.12609600891469458No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.12609600891469458No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA430.12609600891469458No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA430.12609600891469458No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.12609600891469458No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC430.12609600891469458No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC420.12316354359109703No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA420.12316354359109703No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.1202310782674995No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.11729861294390194No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT390.11436614762030438No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT390.11436614762030438No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT390.11436614762030438No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA380.11143368229670685No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCC370.10850121697310929No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA370.10850121697310929No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA370.10850121697310929No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA370.10850121697310929No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG360.10556875164951175No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC360.10556875164951175No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT360.10556875164951175No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT360.10556875164951175No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC360.10556875164951175No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT350.10263628632591419No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC350.10263628632591419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)