FastQCFastQC Report
Sun 21 May 2023
EGAF00007838375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34362
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA20555.980443513183167No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA13954.0597171293871135No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11243.271055235434492No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10803.1430068098480883No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8502.4736627670100693No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA8112.360165298876666No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7252.109888830685059No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6992.034223851929457No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6992.034223851929457No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6651.9352773412490543No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5851.7024620220010476No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5701.6588091496420463No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5651.644258192189046No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5601.6297072347360455No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5161.5016588091496421No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5081.4783772772248414No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5081.4783772772248414No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4721.3736103835632385No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4461.2979454048076364No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4291.248472149467435No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4241.2339211920144346No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3851.1204237238810315No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3711.0796810430126302No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3611.0505791281066295No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3521.0243874046912287No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3511.0214772132006287No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.9516326174262266No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3150.9167103195390257No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT2970.8643268727082243No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2940.8555962982364239No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2830.8235841918398231No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2830.8235841918398231No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2780.8090332343868226No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2720.7915720854432222No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.7799313194808218No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2610.7595599790466213No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2450.7129969151970199No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2240.6518828938944182No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.643152319422618No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2080.605319830044817No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2010.5849484896106163No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1990.5791281066294163No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.5762179151388162No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.5616669576858158No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1920.5587567661952156No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1880.5471160002328154No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1850.5383854257610151No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1810.5267446597986147No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1800.5238344683080147No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.5209242768174146No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.4685408299866131No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1590.4627204470054129No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1550.45107968104301266No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.42488795762761195No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1380.4016064257028112No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1370.39869623421221123No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1370.39869623421221123No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1360.3957860427216111No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1320.38414527675921073No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1290.37541470228741053No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1220.35504336185320995No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1210.3521331703626099No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT1190.3463127873814097No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1060.30848029800360866No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.3026599150224085No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1030.2997497235318084No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1020.29683953204120833No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1010.2939293405506082No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT990.2881089575694081No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT980.285198766078808No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT920.2677376171352075No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT880.25609685117280717No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.25027646819160704No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT860.25027646819160704No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT840.24445608521040685No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT830.24154589371980675No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA820.23863570222920666No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC820.23863570222920666No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA800.23281531924800652No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT750.2182643617950061No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA750.2182643617950061No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT740.215354170304406No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT730.21244397881380597No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA690.2008032128514056No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT670.19498282987020546No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT650.1891624468890053No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT650.1891624468890053No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.1891624468890053No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.16879110645480472No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG570.16588091496420465No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA520.15132995751120426No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG510.14841976602060417No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA510.14841976602060417No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT510.14841976602060417No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA510.14841976602060417No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT500.14550957453000407No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.14550957453000407No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA490.142599383039404No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA490.142599383039404No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA480.1396891915488039No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA480.1396891915488039No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA470.13677900005820384No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT470.13677900005820384No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.13386880856760375No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC460.13386880856760375No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA460.13386880856760375No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA450.13095861707700368No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT430.12513823409580352No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT430.12513823409580352No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCT420.12222804260520342No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT420.12222804260520342No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.11931785111460333No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11640765962400326No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC390.11349746813340317No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.1105872766428031No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC380.1105872766428031No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA380.1105872766428031No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA370.107677085152203No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA370.107677085152203No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC370.107677085152203No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.107677085152203No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.10476689366160294No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT360.10476689366160294No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA360.10476689366160294No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC360.10476689366160294No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC350.10185670217100284No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA350.10185670217100284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)