FastQCFastQC Report
Sun 21 May 2023
EGAF00007838385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36884
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA17824.8313631927122875No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12793.467628239887214No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9822.6624010411018326No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8862.402125582908578No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG8862.402125582908578No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8832.393991974840039No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6651.80294978852619No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6291.7053464917037198No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6171.672812059429563No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5741.556230343780501No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5551.5047174926797529No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5201.4098253985467954No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4961.3447565339984817No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4821.3067996963452988No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4611.2498644398655243No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4591.2444420344864981No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4371.1847955753172106No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4191.1359939269059756No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3701.0031449951198352No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3691.0004337924303222No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.9814553736037306No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3470.9407873332610346No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3430.9299425225029823No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3340.9055416982973647No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3210.8702960633336948No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3150.8540288471966164No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2980.8079384014748942No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2860.7754039692007374No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.7618479557531721No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2810.7618479557531721No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2670.7238911180999892No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2670.7238911180999892No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2580.6994902938943716No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2550.6913566858258323No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2460.6669558616202147No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2450.6642446589307017No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2420.6561110508621624No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2370.6425550374145972No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2350.637132632035571No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.6100206051404403No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.6100206051404403No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.5774861728662835No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2080.5639301594187182No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2080.5639301594187182No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2060.558507754039692No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2030.5503741459711528No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1930.5232621190760222No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1910.517839713696996No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1870.5069949029389437No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1710.4636156599067346No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1690.45819325452770854No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.4527708491486824No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1630.4419260383906301No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.4148140114954994No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1470.398546795358421No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.3958355926689079No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1450.39312438997939486No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1440.3904131872898818No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.3822795792213426No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1400.3795683765318295No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1340.36330116039475113No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1300.35245634963669886No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1280.3470339442576727No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.3416115388786466No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1220.33076672812059427No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.3280555254310812No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1200.32534432274156816No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1190.3226331200520551No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1180.319921917362542No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1170.3172107146730289No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.29823229584643746No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1060.2873874850883852No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1030.279253877019846No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1020.27654267433033297No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.2656978635722807No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT980.2656978635722807No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT970.2629866608827676No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.26027545819325454No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA960.26027545819325454No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT910.24671944474568916No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.23858583667714997No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC880.23858583667714997No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT860.23316343129812386No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA840.2277410259190977No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT820.22231862054007157No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA820.22231862054007157No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA820.22231862054007157No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC810.2196074178505585No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT800.21689621516104543No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.2114738097820193No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT770.2087626070925062No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA760.2060514044029932No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG750.2033402017134801No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.20062899902396703No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA730.19791779633445394No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT720.1952065936449409No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.19249539095542784No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.19249539095542784No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT710.19249539095542784No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.18978418826591475No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.18436178288688862No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT670.18165058019737557No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT640.17351697212883635No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.16809456674981021No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.16538336406029713No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT590.159960958681271No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT580.15724975599175794No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.15724975599175794No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA570.15453855330224486No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA570.15453855330224486No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT570.15453855330224486No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC570.15453855330224486No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC570.15453855330224486No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA570.15453855330224486No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.1518273506127318No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.1518273506127318No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1518273506127318No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC560.1518273506127318No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC550.14911614792321873No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA550.14911614792321873No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT550.14911614792321873No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC540.14640494523370567No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.1436937425441926No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC520.14098253985467954No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT510.13827133716516649No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT510.13827133716516649No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC510.13827133716516649No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT500.1355601344756534No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA490.13284893178614035No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT470.12742652640711422No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT470.12742652640711422No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT450.12200412102808807No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.12200412102808807No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.11929291833857499No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.11929291833857499No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT440.11929291833857499No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC440.11929291833857499No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT430.11658171564906193No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT430.11658171564906193No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT430.11658171564906193No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA420.11387051295954885No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.11387051295954885No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT410.11115931027003578No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.11115931027003578No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.11115931027003578No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA400.10844810758052272No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC400.10844810758052272No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT400.10844810758052272No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA390.10573690489100965No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG390.10573690489100965No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT390.10573690489100965No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA390.10573690489100965No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.1030257022014966No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA380.1030257022014966No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC370.10031449951198351No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT370.10031449951198351No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG370.10031449951198351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)