FastQCFastQC Report
Sun 21 May 2023
EGAF00007838391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25992
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG7943.054786088027085No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6642.5546321945213912No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6102.3468759618344106No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6042.3237919359803016No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5121.9698368728839641No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5001.9236688211757464No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3991.5350877192982455No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3991.5350877192982455No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3691.4196675900277007No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3521.3542628501077256No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3481.3388734995383196No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3281.2619267466912896No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3231.2426900584795322No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3001.154201292705448No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2951.1349646044936903No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2871.1041859033548784No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2821.084949215143121No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2761.0618651892890119No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2711.0426285010772545No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2701.0387811634349031No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2611.0041551246537395No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2480.9541397353031703No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2360.9079716835949523No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2350.9041243459526008No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2340.9002770083102494No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2210.8502616189596799No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2190.8425669436749769No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2080.8002462296091105No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2070.7963988919667591No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1970.757925515543244No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1960.7540781779008926No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1880.7232994767620806No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.7194521391197292No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1870.7194521391197292No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.6886734379809172No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1760.6771314250538628No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1750.6732840874115112No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1710.6578947368421052No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1700.6540473991997537No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1680.6463527239150507No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.6155740227762388No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.5886426592797784No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1530.5886426592797784No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.5732533087103724No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1480.5694059710680209No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1470.5655586334256694No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1470.5655586334256694No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.546321945213912No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1310.5040012311480455No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1290.4963065558633426No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1290.4963065558633426No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.4963065558633426No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.49245921822099104No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.48476454293628807No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.4809172052939366No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1230.4732225300092336No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1210.4655278547245306No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.4501385041551247No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1140.43859649122807015No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1130.4347491535857187No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1120.43090181594336713No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1090.4193598030163127No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1090.4193598030163127No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1070.4116651277316097No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT990.3808864265927978No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA980.3770390889504463No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA900.3462603878116343No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT900.3462603878116343No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA890.34241305016928286No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.3347183748845799No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC860.3308710372422284No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA850.32702369959987687No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.3193290243151739No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT800.3077870113881194No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.3039396737457679No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT790.3039396737457679No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA790.3039396737457679No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.30009233610341646No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT770.29624499846106495No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT770.29624499846106495No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.29239766081871343No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT740.28470298553401047No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.28085564789165895No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA730.28085564789165895No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT700.2693136349646045No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA700.2693136349646045No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA690.26546629732225296No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT680.2616189596799015No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA670.25777162203755005No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT650.25007694675284703No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT640.24622960911049552No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT640.24622960911049552No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.22699292089873807No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT590.22699292089873807No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA570.21929824561403508No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT570.21929824561403508No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.21929824561403508No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.21545090797168356No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA560.21545090797168356No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.2039088950446291No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA530.2039088950446291No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA520.20006155740227763No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT510.19621421975992612No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA500.19236688211757463No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.19236688211757463No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.19236688211757463No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA490.18851954447522315No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC490.18851954447522315No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT490.18851954447522315No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC480.18467220683287164No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA470.18082486919052015No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.1692828562634657No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA420.16158818097876268No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT420.16158818097876268No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC420.16158818097876268No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC420.16158818097876268No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT420.16158818097876268No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.1577408433364112No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT400.1538935056940597No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT390.15004616805170823No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT390.15004616805170823No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA390.15004616805170823No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA390.15004616805170823No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA380.14619883040935672No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA380.14619883040935672No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.14619883040935672No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC380.14619883040935672No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC380.14619883040935672No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC380.14619883040935672No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT380.14619883040935672No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA370.14235149276700523No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA360.13850415512465375No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCT360.13850415512465375No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT360.13850415512465375No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC360.13850415512465375No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT360.13850415512465375No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT340.13080947983995075No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCT340.13080947983995075No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT330.12696214219759927No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA330.12696214219759927No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.12696214219759927No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA320.12311480455524776No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCT320.12311480455524776No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG320.12311480455524776No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT320.12311480455524776No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA320.12311480455524776No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT320.12311480455524776No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT310.11926746691289629No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA310.11926746691289629No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC310.11926746691289629No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA310.11926746691289629No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC300.11542012927054479No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA290.1115727916281933No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA280.10772545398584178No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT280.10772545398584178No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA280.10772545398584178No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT280.10772545398584178No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT260.10003077870113881No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA260.10003077870113881No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA260.10003077870113881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)