FastQCFastQC Report
Sun 21 May 2023
EGAF00007838396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32055
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18195.67462174387771No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16245.066292310092029No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9172.8607081578536886No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8832.754640461706442No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6672.0807986273592265No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5501.7158009670878178No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5461.7033224145999064No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5311.6565278427702386No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5181.6159725471845268No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5111.5941350803306817No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5031.5691779753548587No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4971.5504601466229917No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4901.528622679769147No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4801.4974262985493683No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3881.210419591327406No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3631.1324286382779598No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3441.0731555139603806No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3281.023241304008735No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3150.9826860084230229No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3120.9733270940570894No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3120.9733270940570894No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3100.9670878178131337No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.9546092653252223No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3000.9358914365933552No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2920.9109343316175325No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2430.7580720636406177No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2350.7331149586647948No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.7175167680549056No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.7175167680549056No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2270.7081578536889721No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2160.6738418343472158No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1990.6208079862735922No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1990.6208079862735922No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1910.5958508812977694No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1840.5740134144439245No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1820.5677741381999688No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1810.5646545000779909No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1790.5584152238340353No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1760.5490563094681017No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1740.542817033224146No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1710.5334581188582125No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1690.5272188426142567No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1600.4991420995164561No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.48978318515052255No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1490.46482608017469973No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1470.458586803930744No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1460.45546716580876623No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.44922788956481047No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1420.44298861332085476No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1380.43051006083294335No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.4273904227109655No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.42115114646700985No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.42115114646700985No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1270.39619404149118703No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1250.3899547652472313No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1240.3868351271252535No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.3837154890032756No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1230.3837154890032756No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1200.37435657463734207No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1200.37435657463734207No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1190.3712369365153642No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.3712369365153642No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1180.36811729839338636No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.35563874590547495No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1140.35563874590547495No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.34316019341756354No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.33380127905163No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1060.3306816409296522No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1050.32756200280767434No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1020.3182030884417408No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.31508345031976287No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT980.30572453595382937No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA970.3026048978318515No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT950.2963656215878958No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA930.2901263453439401No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT920.28700670722196225No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC870.271408516612073No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT860.26828887849009514No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC840.2620496022461395No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT840.2620496022461395No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.2589299641241616No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.2589299641241616No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA820.25581032600218373No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT820.25581032600218373No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.25581032600218373No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT790.24645141163625017No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT780.24333177351427235No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT770.2402121353922945No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.22773358290438311No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG730.22773358290438311No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC700.21837466853844956No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT700.21837466853844956No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT690.21525503041647168No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA680.21213539229449382No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC670.209015754172516No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.20277647792856027No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT650.20277647792856027No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC640.19965683980658244No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.19965683980658244No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA640.19965683980658244No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG630.1965372016846046No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.1965372016846046No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT630.1965372016846046No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.19341756356262674No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.19341756356262674No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA620.19341756356262674No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT610.19029792544064889No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.19029792544064889No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC590.18405864919669318No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC590.18405864919669318No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC580.18093901107471533No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT580.18093901107471533No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT570.17781937295273748No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.17469973483075962No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA550.17158009670878177No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT540.16846045858680395No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA540.16846045858680395No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA530.1653408204648261No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.1653408204648261No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.1622211823428482No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.1591015442208704No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA510.1591015442208704No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT500.15598190609889254No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA490.15286226797691468No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT480.14974262985493683No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.14662299173295898No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.14662299173295898No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.14662299173295898No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.14350335361098113No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT460.14350335361098113No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT460.14350335361098113No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC460.14350335361098113No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT460.14350335361098113No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG460.14350335361098113No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC450.14038371548900327No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.13726407736702542No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT430.13414443924504757No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA430.13414443924504757No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC410.12790516300109186No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT400.12478552487911403No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT380.11854624863515831No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT380.11854624863515831No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT380.11854624863515831No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA370.11542661051318047No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.11230697239120262No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA360.11230697239120262No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT350.10918733426922478No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT350.10918733426922478No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG350.10918733426922478No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT350.10918733426922478No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA350.10918733426922478No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA340.10606769614724691No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA340.10606769614724691No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10606769614724691No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA340.10606769614724691No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT340.10606769614724691No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC340.10606769614724691No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC330.10294805802526907No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT330.10294805802526907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)