FastQCFastQC Report
Sun 21 May 2023
EGAF00007838397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32055
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA17785.546716580876619No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16545.159881453751365No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9012.8107939479020434No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9002.8076743097800656No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6902.152550304164717No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5361.672126033380128No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5361.672126033380128No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4931.5379815941350803No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4891.5255030416471689No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4711.4693495554515676No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4641.4475120885977226No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4611.438153174231789No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4491.4007175167680548No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4031.257214163157074No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3681.148026828887849No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3381.0544376852285133No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3321.0357198564966463No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3231.0076431133988457No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3200.9982841990329122No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.960848541569178No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3010.9390110747153331No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2970.9265325222274216No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2970.9265325222274216No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2920.9109343316175325No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2890.9015754172515988No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.770550616128529No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2420.7549524255186398No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.6925596630790829No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2100.6551240056153487No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.6145687100296365No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.6114490719076587No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.6020901575417251No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1900.5927312431757916No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1900.5927312431757916No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1850.5771330525659024No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1770.5521759475900796No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1760.5490563094681017No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1750.5459366713461238No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1730.5396973951021682No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1710.5334581188582125No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1650.5147402901263454No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.5147402901263454No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1610.502261737638434No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1580.49290282327250035No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1540.480424270784589No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.47418499454063323No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1490.46482608017469973No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1490.46482608017469973No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1460.45546716580876623No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1460.45546716580876623No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1400.4367493370768991No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1370.4273904227109655No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.42115114646700985No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1340.418031508345032No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1310.4086725939790984No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1270.39619404149118703No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1240.3868351271252535No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1210.3774762127593199No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1200.37435657463734207No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1190.3712369365153642No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1180.36811729839338636No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1180.36811729839338636No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1170.3649976602714085No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1160.36187802214943066No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.35563874590547495No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.35563874590547495No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1130.3525191077834971No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1090.34004055529558574No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1060.3306816409296522No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1040.3244423646856964No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.31508345031976287No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1000.3119638121977851No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.30572453595382937No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT960.29948525970987366No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT960.29948525970987366No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA950.2963656215878958No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC950.2963656215878958No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT920.28700670722196225No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC910.2838870690999844No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA890.2776477928560287No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT880.27452815473405084No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.271408516612073No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT860.26828887849009514No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.2589299641241616No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA800.24957104975822805No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT800.24957104975822805No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT770.2402121353922945No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.23709249727031662No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC750.2339728591483388No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT730.22773358290438311No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.22149430666042738No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA710.22149430666042738No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.21837466853844956No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.21525503041647168No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.21525503041647168No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA680.21213539229449382No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC670.209015754172516No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA660.20589611605053815No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC650.20277647792856027No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.20277647792856027No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG650.20277647792856027No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG650.20277647792856027No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC640.19965683980658244No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC630.1965372016846046No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT620.19341756356262674No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA620.19341756356262674No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.19341756356262674No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.19341756356262674No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT610.19029792544064889No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.19029792544064889No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.18405864919669318No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA570.17781937295273748No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA570.17781937295273748No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA550.17158009670878177No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT540.16846045858680395No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT540.16846045858680395No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA530.1653408204648261No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA530.1653408204648261No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT520.1622211823428482No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.1622211823428482No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA520.1622211823428482No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.1591015442208704No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.15598190609889254No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC500.15598190609889254No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.15286226797691468No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA480.14974262985493683No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.14974262985493683No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT480.14974262985493683No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT480.14974262985493683No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT460.14350335361098113No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.14038371548900327No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT450.14038371548900327No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT450.14038371548900327No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA440.13726407736702542No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC440.13726407736702542No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT440.13726407736702542No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT430.13414443924504757No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC430.13414443924504757No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.13102480112306975No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA420.13102480112306975No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA420.13102480112306975No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA410.12790516300109186No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT410.12790516300109186No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT400.12478552487911403No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT390.12166588675713617No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT390.12166588675713617No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.12166588675713617No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT390.12166588675713617No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.11854624863515831No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC380.11854624863515831No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA380.11854624863515831No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.11854624863515831No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA380.11854624863515831No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT380.11854624863515831No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC380.11854624863515831No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT370.11542661051318047No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT360.11230697239120262No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC360.11230697239120262No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA350.10918733426922478No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA350.10918733426922478No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.10918733426922478No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT350.10918733426922478No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT350.10918733426922478No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA350.10918733426922478No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA340.10606769614724691No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT340.10606769614724691No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT340.10606769614724691No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC330.10294805802526907No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT330.10294805802526907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)