FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007838788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56336436
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23714074.209366385903432No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG19273653.421169560672954TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG3897350.6917991759365112TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1216190.215879825979762TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG1084910.1925769674176762TruSeq Adapter, Index 6 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGG821420.14580617062818813No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGG579200.10281090553900143TruSeq Adapter, Index 6 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)