FastQCFastQC Report
Thu 12 Jan 2023
EGAF00007838790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42312889
Sequences flagged as poor quality0
Sequence length75
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG31524357.4502948735076915TruSeq Adapter, Index 11 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10096582.3861712680502625No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG6978501.6492610561287837TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1981980.4684104647167912TruSeq Adapter, Index 11 (100% over 63bp)
TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAT1551590.36669441313733037No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG1477880.3492741892429042TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1254330.2964415878102769TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1141990.26989175804091275TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGG1037400.24517352147710833TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG1033440.24423763643271912TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG917810.21691026580576903TruSeq Adapter, Index 11 (100% over 63bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTT721280.17046342545884777TruSeq Adapter, Index 11 (100% over 56bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG721270.1704610621127761TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAAGG692590.16368298557916952TruSeq Adapter, Index 11 (98% over 63bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC688040.16260766311655062TruSeq Adapter, Index 11 (100% over 54bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAAGG605860.14318568509940316TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAGGG540900.1278333890177057TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGG510180.12057318988547437TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT498570.11782934509624243TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG495890.11719596834902954TruSeq Adapter, Index 11 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)