FastQCFastQC Report
Sun 5 May 2024
EGAF00007838862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467517819
Total Bases70.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC55504471.1872161390280613Illumina Single End PCR Primer 1 (96% over 33bp)
AGTGATTAGCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT12126420.25937877674775855No Hit
AGTGATTAGCATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA9462520.2023991303741088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)