FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007838903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517801152
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAATTATCTCGTATG56303571.0873589172702343TruSeq Adapter, Index 5 (97% over 36bp)
CCAAGATCTGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT27571270.5324683016541454No Hit
CCAAGATCTGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA17251130.33316129045614795No Hit
CCAAGATCTGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC8193340.15823332892082867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)