FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59812
Sequences flagged as poor quality0
Sequence length35-151
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA13492.255400254129606No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8621.4411823714304823No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA7001.1703337122985353No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA6041.0098308031833076No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5520.9228917274125593No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA4520.7557011970841971No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4410.7373102387480773No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4240.7088878485922557No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3700.6186049622149401No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.6169330569116566No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.5550725606901624No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA3200.5350096970507591No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA3100.5182906440179228No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA2900.4848525379522504No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA2770.46311776900956325No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.4046010833946365No Hit
ATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2340.3912258409683676No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2340.3912258409683676No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA2070.34608439777970973No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA2020.33772487126329165No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAA2010.33605296596000805No Hit
GTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.322677723533739No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA1700.28422390155821575No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1630.2725205644352304No Hit
GTACATGGGAAGCAGTGGTATCAAGCAGTGGTAAAAAAAAAAAAAAAAAA1580.2641610379188123No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAA1570.26248913261552864No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA1330.22236340533672172No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1320.22069150003343813No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1290.21567578412358723No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAA1280.2140038788203036No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1180.1972848257874674No Hit
ACGCAGAGTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAA1160.19394101518090015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.1905972045743329No Hit
GTACATGGGAAGCAGTGGTATCAAGCAGTGGAAAAAAAAAAAAAAAAAAA1100.18390958336119842No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA1070.17889386745134755No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA1040.1738781515414967No Hit
GTACATGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.1738781515414967No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1030.17220624623821307No Hit
GTATCAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.17220624623821307No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAA1010.16886243563164582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG930.15548719320537685No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCAGTGGTAAAAAA880.1471276666889587No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAA850.14211195077910788No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA850.14211195077910788No Hit
GCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC840.14044004547582425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG790.13208051895940615No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCAGTGGTAAAAA780.1304086136561225No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGC770.1287367083528389No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.12706480304955528No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAA730.1220490871397044No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.12037718183642078No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.12037718183642078No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11870527653313717No Hit
CAGTGGTATCAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11703337122985355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.11536146592656991No Hit
GTATCAACGCAGAGTACATGGGTATCAACGCAAAAAAAAAAAAAAAAAAA690.11536146592656991No Hit
TCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA690.11536146592656991No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAA680.11368956062328629No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAGCAGTGGTAAAAAAAAAAA680.11368956062328629No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG670.11201765532000267No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA650.10867384471343544No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA640.1070019394101518No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAA640.1070019394101518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG620.10365812880358456No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA620.10365812880358456No Hit
ATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA610.10198622350030095No Hit
GTATCAAGCAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10031431819701733No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA600.10031431819701733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)