FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3934005
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC227170.5774522401471275No Hit
GAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTAC189120.4807314683128262No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT169830.43169746861023306No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAAC165190.41990287251795555No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGT152740.3882557342962198No Hit
GTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGA142580.3624296359562329No Hit
TCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGC118990.30246529935777916No Hit
ATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCC114630.29138244613313913No Hit
GTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCC114160.29018773489103344No Hit
CACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC113480.28845921649819967No Hit
TAGTTGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT104930.26672563964712803No Hit
GTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCT98700.25088936084219515No Hit
CCCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGA97830.24867787407489317No Hit
CCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGAC95970.2439498678827302No Hit
TTCCAGAGCGTTCCGCTAATCTCAAAGCCACTTAAGGGCTAGTCCCCGTT95700.24326354440322268No Hit
GTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTC91440.2324348850598817No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA90380.22974042991811142No Hit
TCATAAGATGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCA88660.2253682951597672No Hit
TATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGAT85940.21845422158843214No Hit
CTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT83600.21250608476603358No Hit
TATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATT82820.21052337249190073No Hit
CTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTG81930.2082610469483389No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG79940.20320258871048716No Hit
GATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAA79790.20282129788853853No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAA78120.1985762600708438No Hit
GTATTAATCTACAACCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA77050.19585638554094364No Hit
GTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTATCACC76960.19562761104777449No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTT76340.19405160898372015No Hit
GAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAG75730.19250102630779575No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGT75580.19211973548584713No Hit
GTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTA71920.18281623943030068No Hit
CACCAACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC67260.1709708045617634No Hit
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATC64210.16321789118214136No Hit
GTACATGGGGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG63160.16054885542850098No Hit
CCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCA62330.1584390462137186No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAA59600.15149955325425363No Hit
GTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGT56690.1441025113084503No Hit
TAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT56250.14298405823073435No Hit
TAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAG55940.14219605719870718No Hit
CGGTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATG55750.14171308882423891No Hit
GTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA55630.14140805616668No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAGCTGTCTAAGCCTTC55300.14056921635839303No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTA55020.1398574734907556No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGACACGTCGAGCAGGT54740.13914573062311816No Hit
CAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTT54510.13856108469613027No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCCAGTTCGCGTGGAG52890.13444314381908512No Hit
TAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGA52040.1322824958280429No Hit
ACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTT51840.13177410806544476No Hit
ACCATACTCTAGTCAGTCAGTTTTGAATGCAGTTCCCAGGTTGAGCCCGG51460.13080817131650824No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGGTTAAGCTCGCCACT49690.12630893961751447No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGAT49390.1255463579736172No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGCTTGTAC48240.12262312833867775No Hit
TTTCAACGGTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAA47280.12018286707820655No Hit
TAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGA47260.12013202830194675No Hit
GGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGAGGAA46570.11837809052098307No Hit
TTATCGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCGTT46360.117844283370255No Hit
ATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCC46030.11700544356196801No Hit
TAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGA45640.11601408742490159No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTG45350.11527692516913425No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAA45240.11499731189970526No Hit
GTAGAGAGAAGCTTGCTTCTCTTGAGAGCGGCGGACGGGTGAGTAATGCC45020.11443808536084728No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTACTGACTGACCGATA44020.11189614654785644No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGCTTAACCGA43320.11011678937876286No Hit
ACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAG42710.10856620670283847No Hit
GCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAG42620.1083374322096693No Hit
GTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTC42140.1071173015794337No Hit
TTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCGTT41470.10541420257472983No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGAT41460.10538878318659992No Hit
TCTCAAAGCCACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGG41250.10485497603587185No Hit
ATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGAC40970.1041432331682344No Hit
ATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCT40420.10274516682108946No Hit
CCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAG40350.10256723110418009No Hit
ATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAG39950.10155045557898375No Hit
GTCTGAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAAT39920.10147419741459403No Hit
GAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAATACAT39850.10129626169768469No Hit
CATCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTG39740.10101664842825568No Hit
GCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCAT39620.10071161577069679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)