FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854819
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC318670.8266795405958101No Hit
GAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTAC238440.6185504429650264No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT236380.6132064825871202No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGT216870.5625945083284065No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAAC206360.535329933778992No Hit
GTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGA204350.5301156811772486No Hit
GTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCC167930.43563653702028554No Hit
CACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC154520.4008489114534301No Hit
TCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGC148540.3853358614243626No Hit
ATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCC143420.37205378514529475No Hit
GTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCT135570.35168966428773957No Hit
CCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGAC128790.3341012898400677No Hit
GTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTC128760.3340234651743701No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA128390.3330636276307655No Hit
TTCCAGAGCGTTCCGCTAATCTCAAAGCCACTTAAGGGCTAGTCCCCGTT126270.32756401792146406No Hit
CTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT123540.3204819733429767No Hit
TAGTTGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT120890.31360746120634975No Hit
TATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGAT120530.3126735652179778No Hit
CCCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGA119380.3096902863662341No Hit
TATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATT114050.29586343742728255No Hit
TCATAAGATGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCA110810.287458373531935No Hit
GAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAG107140.2779378227615875No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGT105180.27285327793600683No Hit
CTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTG104370.2707520119621699No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAA101010.2620356494040317No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTT99630.2584557147819392No Hit
GTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTATCACC98840.2564063319185674No Hit
CACCAACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC91130.23640539283426798No Hit
GTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTA90130.2338112373110125No Hit
CCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCA85090.22073669347380515No Hit
GTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGT79740.20685796142438853No Hit
CGGTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATG78350.20325208524706348No Hit
GTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA77860.20198094904066832No Hit
GATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAA75580.19606627444764593No Hit
GTATTAATCTACAACCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA73780.19139679450578614No Hit
CAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTT73080.1895808856395073No Hit
ACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTT72440.18792062610462384No Hit
TAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAG70130.1819281268459038No Hit
TAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT63990.16600001193311542No Hit
GTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTC62650.16252384353195312No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGAT62210.16138241510172072No Hit
ACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAG61570.15972215556683725No Hit
ATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCC59740.1549748509592798No Hit
GGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGAGGAA59030.15313300053776843No Hit
GTACATGGGGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG58550.15188780588660583No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGAT57610.1494492996947457No Hit
ATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCT57180.1483338128197459No Hit
TAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGA57090.1481003388226529No Hit
GCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAG57000.14786686482555988No Hit
TCTCAAAGCCACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGG54790.1421337811191654No Hit
AGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAG53870.13974715803777038No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTA53840.1396693333720727No Hit
ATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGAC53330.13834631405521244No Hit
GTCTGAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAAT52770.1368935869621894No Hit
GGTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGG52170.13533709364823615No Hit
GGGCTATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCA51830.1344550807703293No Hit
TATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGACTAAG51400.13333959389532946No Hit
GAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAATACAT50800.1317831005813762No Hit
ACCATACTCTAGTCAGTCAGTTTTGAATGCAGTTCCCAGGTTGAGCCCGG50560.1311605032557949No Hit
CATCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTG50450.13087514614823678No Hit
GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTAT48860.12675043886626064No Hit
ATGAAAGACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGAC47860.1241562833430052No Hit
CTCGTGCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAG47820.12405251712207499No Hit
GCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCAT47470.12314456268893559No Hit
GAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG47280.12265167313951705No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG46640.12099141360463357No Hit
CGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGC46320.12016128383719185No Hit
TGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACT46060.11948680340114542No Hit
AGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGAT43780.11357212880812302No Hit
GTAGAGAGAAGCTTGCTTCTCTTGAGAGCGGCGGACGGGTGAGTAATGCC42810.11105579795056525No Hit
GAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGAGCGAACGGGG42740.11087420706393737No Hit
AACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAA42530.11032943440405373No Hit
GTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTC42320.10978466174417008No Hit
CATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGA42300.109732778633705No Hit
ATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAGCTTTT41630.10799469443312384No Hit
ACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTT40690.10555618824126373No Hit
TCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCTG40580.10527083113370563No Hit
TCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGT40530.10514112335754287No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTG40090.10399969492731047No Hit
CTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACAT39980.10371433781975235No Hit
GTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCG39950.10363651315405471No Hit
GTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGA39350.10208001984010144No Hit
ACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGACTTTGATC38680.1003419356395203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)