FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854819
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC326390.8467064212353421No Hit
GAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTAC242190.6282785261772342No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT239110.6202885271656076No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGT218980.5680681764824755No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAAC213430.5536706133284078No Hit
GTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGA213010.5525810680086406No Hit
GTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCC175450.4551445865551664No Hit
TCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGC145980.3786948232848287No Hit
CACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC141870.36803284408424886No Hit
ATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCC138600.35954995552320357No Hit
GTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCT136860.35503612491273906No Hit
GTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTC128220.33262262119181213No Hit
CCCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGA125530.325644342834255No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA125130.32460668062495285No Hit
CCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGAC124760.32364684308134833No Hit
TAGTTGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT121470.31511207140983793No Hit
TATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGAT120800.3133739872092568No Hit
TTCCAGAGCGTTCCGCTAATCTCAAAGCCACTTAAGGGCTAGTCCCCGTT120310.3121028510028616No Hit
CTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT118850.30831538393890867No Hit
TCATAAGATGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCA111490.2892223992877487No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAA109720.28463074401158656No Hit
TATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATT109040.28286671825577286No Hit
GAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAG106620.27658886188949466No Hit
GTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTATCACC104420.27088171973833275No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGT104330.27064824574123975No Hit
CTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTG100800.26149087674414806No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTT96240.24966152755810325No Hit
GTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTA94830.2460037682703131No Hit
CACCAACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC85530.22187812190403752No Hit
CCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCA83120.2156262070929919No Hit
GTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA81040.2102303636046206No Hit
CGGTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATG78760.20431568901159822No Hit
GTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGT77300.20052822194764527No Hit
GTATTAATCTACAACCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA75950.19702611199125045No Hit
GATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAA74630.19360182670055326No Hit
ACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTT71030.18426286681683368No Hit
CAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTT70750.18353650327032217No Hit
TAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAG70050.18172059440404337No Hit
GGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGAGGAA66050.1713439723110216No Hit
TAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT65150.16900923234009171No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGAT63760.16540335616276666No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGAT62300.16161588909881372No Hit
GTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTC62050.16096735021799985No Hit
ACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAG61030.1583213115842793No Hit
GTACATGGGGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG60650.15733553248544224No Hit
ATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCC57680.1496308905813736No Hit
GCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAG57310.1486710530377691No Hit
GGGCTATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCA57050.14799657260172266No Hit
TAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGA56790.14732209216567627No Hit
ATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCT56380.14625848840114153No Hit
ATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGAC55370.14363839132265355No Hit
GTCTGAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAAT55090.14291202777614204No Hit
GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTAT53340.138372255610445No Hit
GGTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGG53020.13754212584300327No Hit
TCTCAAAGCCACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGG52710.13673793763079406No Hit
AGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAG52200.13541491831393382No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTA52170.13533709364823615No Hit
CATCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTG51570.13378060033428288No Hit
TGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACT51220.13287264590114348No Hit
CTCGTGCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAG50270.1304081981540508No Hit
TATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGACTAAG50180.13017472415695783No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG50120.1300190748255625No Hit
ACCATACTCTAGTCAGTCAGTTTTGAATGCAGTTCCCAGGTTGAGCCCGG49450.12828099062498136No Hit
GCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCAT49180.1275805686337024No Hit
GAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAATACAT49170.12755462707846982No Hit
ATGAAAGACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGAC48180.12498641311044696No Hit
GAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG46110.1196165111773082No Hit
GAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGAGCGAACGGGG44540.11554368700579716No Hit
CGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGC43510.11287170681684405No Hit
CATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGA42980.11149680438951867No Hit
CTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACAT42500.11025160973835606No Hit
GTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTC42080.10916206441858878No Hit
GTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGACTTTGATCCG41680.1081244022092866No Hit
AGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGAT41670.10809846065405405No Hit
ACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTT41530.10773527888079829No Hit
GTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGA41140.10672355822672869No Hit
TCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGT41120.10667167511626356No Hit
AACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAA40830.10591937001451948No Hit
ATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAGCTTTT40220.10433693514533367No Hit
ACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGACTTTGATC39870.10342898071219427No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTG39000.10117206540696203No Hit
GTAGAGAGAAGCTTGCTTCTCTTGAGAGCGGCGGACGGGTGAGTAATGCC38620.10018628630812498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)