FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339467
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC309630.7135208079701953No Hit
GAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTAC223350.5146945465883253No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT213240.4913967544862076No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGT207340.4778006146837849No Hit
GTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGA202570.4668084813180973No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAAC199380.45945734810288913No Hit
GTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCC155600.35856938190796245No Hit
TCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGC138130.3183109815099412No Hit
ATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCC134400.30971545583824006No Hit
GTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCT130380.30045164532879265No Hit
GTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTC129840.2992072528722997No Hit
CACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC127050.2927778918470863No Hit
CCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGAC122760.28289188510939245No Hit
CCCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGA121170.2792278406541633No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA120080.27671601143642754No Hit
TATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGAT119330.27498768858018735No Hit
TTCCAGAGCGTTCCGCTAATCTCAAAGCCACTTAAGGGCTAGTCCCCGTT116010.26733697940323087No Hit
TAGTTGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT115150.2653551691947421No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAA108390.24977721918383067No Hit
CTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT108380.24975417487908078No Hit
TATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATT107730.2482562950703393No Hit
TCATAAGATGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCA103470.23843942124689507No Hit
GAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAG103000.23735633892365124No Hit
GTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTATCACC102710.23668805408590504No Hit
CTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTG100040.23053522471768997No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGT95100.21915133817125465No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTT91100.20993361627130708No Hit
GTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTA89650.20659219208257604No Hit
CCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCA83850.193226495327652No Hit
GTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA77840.1793768681729807No Hit
CACCAACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC76640.1766115516029964No Hit
GATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAA75430.17382319072826224No Hit
GTATTAATCTACAACCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA74780.17232531091952077No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG74760.17227922231002102No Hit
CGGTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATG72620.16734774109354905No Hit
GTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGT69390.15990443065934135No Hit
TAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAG68310.15741564574635547No Hit
CAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTT66930.15423553169087356No Hit
ACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTT66170.1524841645298835No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGAT65650.15128586068289032No Hit
GGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGAGGAA63440.14619306933316925No Hit
ACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAG61730.14225249322094166No Hit
GTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTC61130.1408698349359495No Hit
TAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT60360.1390954234702096No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGAT59300.13665272716672347No Hit
GTACATGGGGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG59030.136030530938477No Hit
ATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCT57480.1324586637022473No Hit
GTCTGAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAAT56960.1312603598552541No Hit
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATC56830.13096078389350582No Hit
GCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAG55610.1281493787140218No Hit
GGTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGG54890.12649018877203122No Hit
ATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGAC54590.12579885962953513No Hit
TAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGA53940.12430097982079366No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAA53260.12273396709780256No Hit
GGGCTATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCA51970.11976125178506947No Hit
GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTAT51470.118609036547576No Hit
TCTCAAAGCCACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGG50950.11741073270058282No Hit
ATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCC50080.1154058781873442No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTA49790.11473759334959802No Hit
ATGAAAGACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGAC49690.1145071503020993No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGACACGTCGAGCAGGT49390.11381582115960323No Hit
GAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAATACAT48830.11252534009361058No Hit
GCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCAT48780.11241011856986123No Hit
AGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAG48610.11201836538911344No Hit
CATCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTG48600.11199532108436358No Hit
TGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACT47550.10957566908562733No Hit
ACCATACTCTAGTCAGTCAGTTTTGAATGCAGTTCCCAGGTTGAGCCCGG46970.10823909941013493No Hit
TATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGACTAAG46420.10697166264889213No Hit
GAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG45830.10561204866864986No Hit
CTCGTGCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAG45050.10381459289816007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)