FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4196641
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC302020.719670803387757No Hit
GAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTAC233770.5570407380569364No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT229030.5457459906625323No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGT216460.5157934643444603No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAAC205090.4887003677464906No Hit
GTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGA196640.4685652168007699No Hit
GTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCC164760.39259970057005117No Hit
TCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGC151530.36107448790592284No Hit
CACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC146210.34839768281346917No Hit
ATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCC139470.33233721921889436No Hit
GTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCT136630.32556990221465215No Hit
CCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGAC133040.31701544163534595No Hit
TTCCAGAGCGTTCCGCTAATCTCAAAGCCACTTAAGGGCTAGTCCCCGTT130530.31103446780413196No Hit
GTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTC125760.29966823466672515No Hit
TATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGAT122910.2928770890814821No Hit
CCCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGA122780.292567317528471No Hit
TAGTTGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT119870.2856332004572228No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA119770.2853949146472143No Hit
CTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT118360.282035084726094No Hit
CTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTG114480.27278959529776314No Hit
TATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATT112810.26881022227062074No Hit
TCATAAGATGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCA111390.2654265637684996No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGT104380.2487227284869018No Hit
GAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAG102480.24419529809673973No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAA102090.2432659834377065No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTT101300.24138352553863912No Hit
GTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTATCACC100060.23842878149453337No Hit
GTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTA90840.21645882981174708No Hit
CCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCA83820.1997311659491484No Hit
CACCAACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC82390.19632367886602645No Hit
GTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGT77430.18450470268960342No Hit
GATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAA76600.18252693046653265No Hit
CGGTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATG76100.18133550141649No Hit
GTATTAATCTACAACCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA75820.18066830114846613No Hit
CAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTT75450.17978664365143457No Hit
GTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA74380.17723698548434333No Hit
TAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAG73710.17564047055728615No Hit
ACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTT69080.16460783755389133No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGAT65510.15610103413658685No Hit
TAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT65280.15555297677356725No Hit
ATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCC60590.14437737228416725No Hit
ACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAG60490.14413908647415874No Hit
GGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGAGGAA59490.14175622837407345No Hit
GTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTC58840.14020737060901803No Hit
GTACATGGGGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG58340.13901594155897537No Hit
TAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGA58290.13889679865397112No Hit
TCTCAAAGCCACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGG57800.13772919818492935No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTA56190.13389279664379203No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGAT55780.13291582482275707No Hit
ATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCT55330.1318435386777187No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG55120.13134313847670076No Hit
ATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGAC55030.1311286812476931No Hit
GCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAG53980.12862668024260354No Hit
GAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAATACAT53400.1272446225445541No Hit
GTCTGAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAAT53190.12674422234353616No Hit
GGTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGG53080.1264821079525268No Hit
AGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAG52990.12626765072351911No Hit
TATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGACTAAG51540.12281250647839545No Hit
CATCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTG51480.12266953499239035No Hit
ATGAAAGACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGAC50370.12002456250129567No Hit
ACCATACTCTAGTCAGTCAGTTTTGAATGCAGTTCCCAGGTTGAGCCCGG49600.11818976176423No Hit
GGGCTATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCA49170.11716513278119334No Hit
CTCGTGCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAG49150.11711747561919163No Hit
GCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCAT49100.11699833271418739No Hit
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATC48130.11468696035710464No Hit
GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTAT47890.11411507441308419No Hit
GAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG46830.11158924482699377No Hit
TGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACT45830.1092063867269085No Hit
CGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGC45010.10725244308483857No Hit
ACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTT44970.10715712876083515No Hit
ATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAGCTTTT44890.10696650011282832No Hit
GAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGAGCGAACGGGG44550.10615632835879933No Hit
AACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAA44430.1058703853867891No Hit
CATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGA44070.1050125564707584No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTG43780.10432152762173366No Hit
AGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGAT43430.10348752728670382No Hit
GTAGAGAGAAGCTTGCTTCTCTTGAGAGCGGCGGACGGGTGAGTAATGCC42440.10112849776761938No Hit
GTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTC42140.1004136403375938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)