FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215299
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC218600.679874562210233No Hit
GAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTAC171670.5339161303505522No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT163210.5076044249694974No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAAC154230.4796754516453991No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGT150460.4679502590583333No Hit
GTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGA145430.4523063018400466No Hit
GTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCC116890.363543172812233No Hit
CACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC107590.334618957677031No Hit
ATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCC106950.33262847405482354No Hit
TCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGC104840.3260660983628583No Hit
GTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCT100140.31144848426227234No Hit
CCCATATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGAC96940.3014960661512351No Hit
TTCCAGAGCGTTCCGCTAATCTCAAAGCCACTTAAGGGCTAGTCCCCGTT95780.2978883145859841No Hit
GTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTC94400.2935963342755993No Hit
TATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGAT91040.28314629525901014No Hit
CCCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGA86520.26908850467717No Hit
CTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT85220.2650453348195611No Hit
CTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTG85180.26492092959317315No Hit
TAGTTGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT84450.26265053421159273No Hit
TATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATT83680.2602557336036244No Hit
TCATAAGATGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCA82030.25512401801512086No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA81880.254657498416166No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTT78920.2454515116634565No Hit
GTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTATCACC75870.23596561315137413No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGAAA74710.23235786158612312No Hit
GAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAG73270.22787927343615633No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTTCTCGTGCTCCGT68960.21447461029285303No Hit
GTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTA68260.21229751883106363No Hit
CCTATGTATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCA64240.19979479357907304No Hit
CACCAACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCCAC61760.19208166954301917No Hit
GTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGT59330.18452405203995026No Hit
CGGTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATG57070.1774951567490302No Hit
GATTAATACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAA56820.1767176240841054No Hit
GTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA55910.17388740518377918No Hit
GTATTAATCTACAACCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA53730.16710732034563505No Hit
TAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGATTAG52680.16384168315295095No Hit
CAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAGTTT51460.16004732374811798No Hit
ACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTT50790.15796353620611955No Hit
ACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAG45760.14231957898783285No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACTAGCCCTTAAGTGGCTTTGAGAT45480.14144874240311708No Hit
TCTCAAAGCCACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGG44840.13945825878090964No Hit
GTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTC44770.1392405496347307No Hit
TAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAATCGAGT43730.1360060137486436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.1341710366594211No Hit
ATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCAAAGCC41430.12885271323133557No Hit
TAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTGGCGA41180.1280751805664108No Hit
ATTCAGTACAAGATAACCATCTTATGATGGCTGGGTTCCCCCATTCAGAC40450.1258047851848304No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTGAAGAAGCGCATACGGTGGAT40400.12564927865184544No Hit
GCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAG40190.12499615121330863No Hit
GGTGCAAGAGGCGAACCAGGGGAACTGAAACATCTAAGTACCCTGAGGAA39910.12412531462859286No Hit
GAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAATACAT39790.12375209894942897No Hit
GGTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCATACGGTGG39230.12201042577999743No Hit
TATTCAGACAAAGTTTCTCGTGCTCCGTCCTACTCGATTTCATGACTAAG38820.1207352722095208No Hit
GTCTGAATGGGGGAACCCAGCCATCATAAGATGGTTATCTTGTACTGAAT38630.12014434738417795No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTA38050.11834047160155245No Hit
ATCTTATGATGGCTGGGTTCCCCCATTCAGACATCTCCGGATCAAAGTCT37990.11815386376197051No Hit
CATCTAAGTACCCTGAGGAAAAGAAATCAACCGAGATTCCCTTAGTAGTG37840.11768734416301564No Hit
ATGAAAGACGTGGTAGCCTGCGAAAAGCTTCGGGGAGTCGGCAAACAGAC37800.11756293893662767No Hit
AGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAAAG36820.1145150108901225No Hit
GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCTTGCACCTATGTAT35900.1116536906831993No Hit
GGGCTATCACCCACTATGGCCGCACTTTCCAGAGCGTTCCGCTAATCTCA35740.11115606977764743No Hit
GTACATGGGGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG35340.10991201751376777No Hit
GCCCTTAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCAT35110.10919668746203696No Hit
ACCATACTCTAGTCAGTCAGTTTTGAATGCAGTTCCCAGGTTGAGCCCGG34960.1087301678630821No Hit
CTCGTGCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAG34620.1076727234387844No Hit
ACTTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTT34250.1065219750946957No Hit
GAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG33310.1035984522745785No Hit
TGGTTATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACT33070.10285202091625072No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGG32430.10086153729404326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)