FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007839854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007839854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234666001
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2636767911.23625871989867Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25314061.0787272076963548Clontech SMART CDS Primer II A (100% over 24bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8571060.36524507016250723Clontech SMART CDS Primer II A (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6992080.29795879974960665Clontech SMART CDS Primer II A (100% over 25bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5035030.2145615461355222Clontech SMART CDS Primer II A (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3807110.162235261340649No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3737980.15928937230238138Clontech SMART CDS Primer II A (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)