FastQCFastQC Report
Mon 22 May 2023
EGAF00007846507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067192
Sequences flagged as poor quality0
Sequence length54
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCAT2617561.2424816748240581No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA771560.3662377026800724No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC651620.30930557807609105No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGC543290.2578843919968072No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAG435290.20661984758101604No Hit
CTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGAT431650.20489204256552082No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGGGAG396540.18822631891331318No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGGG385510.18299068997899673No Hit
CCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCA352290.16722209585406542No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT327750.15557365215069954No Hit
GCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGA307820.14611344501915585No Hit
CTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGC293530.1393303863182146No Hit
TTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACG291530.13838104290310735No Hit
CCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCA286730.13610261870684998No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCC276310.13115653951414122No Hit
GTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGT275680.13085749633838245No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATT268750.12756802140503587No Hit
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGG266660.12657595753624878No Hit
GGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA258870.12287826493440607No Hit
GCCGGCCGTCGCACTCATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAG249920.11862995315180115No Hit
CGTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCAT245690.11662209182884933No Hit
GTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTG238130.11303357371974394No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCAC237660.11281047801719375No Hit
CCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGC236290.11216017777784529No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228810.1086096334053442No Hit
GCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGG220120.10448473626670322No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCT218780.10384867617858136No Hit
TCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGGGA218030.10349267239791615No Hit
CCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCA212620.10092469846005106No Hit
CTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCC212320.10078229694778498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)