FastQCFastQC Report
Sun 21 May 2023
EGAF00007846646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31600
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21156.693037974683544No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17175.4335443037974684No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6291.9905063291139242No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5931.8765822784810129No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5291.6740506329113922No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5291.6740506329113922No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5141.6265822784810127No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5021.5886075949367089No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4851.5348101265822784No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4541.4367088607594936No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4531.4335443037974684No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4501.4240506329113924No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4221.3354430379746836No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4191.3259493670886076No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4041.2784810126582278No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3951.25No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3771.1930379746835444No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3721.1772151898734178No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3571.1297468354430378No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3521.1139240506329113No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3311.0474683544303798No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3291.0411392405063291No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3211.0158227848101264No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3191.0094936708860758No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3191.0094936708860758No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3150.9968354430379747No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.9746835443037974No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2700.8544303797468354No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2690.8512658227848101No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2620.829113924050633No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2550.8069620253164557No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2510.7943037974683544No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.7278481012658228No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA2290.7246835443037974No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2240.7088607594936709No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA2150.680379746835443No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2150.680379746835443No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.6518987341772152No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1870.5917721518987342No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.560126582278481No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1720.5443037974683544No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1700.5379746835443038No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1650.5221518987341772No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1580.5No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1540.4873417721518987No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.4810126582278481No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1480.46835443037974683No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1430.4525316455696203No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.44936708860759494No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1350.4272151898734177No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1290.40822784810126583No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1250.39556962025316456No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.39556962025316456No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1250.39556962025316456No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1220.3860759493670886No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1220.3860759493670886No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1210.3829113924050633No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1190.37658227848101267No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1180.37341772151898733No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.3670886075949367No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1130.3575949367088607No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1130.3575949367088607No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.3575949367088607No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1090.3449367088607595No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.33860759493670883No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1020.3227848101265823No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.31962025316455694No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT990.31329113924050633No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA970.30696202531645567No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA940.2974683544303797No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT930.29430379746835444No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT920.2911392405063291No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG920.2911392405063291No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.2848101265822785No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT900.2848101265822785No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT870.27531645569620256No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.2721518987341772No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG830.2626582278481013No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG830.2626582278481013No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT810.2563291139240506No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA790.25No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT780.2468354430379747No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT780.2468354430379747No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT770.24367088607594936No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.23734177215189875No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA740.23417721518987342No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA740.23417721518987342No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT700.22151898734177217No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA700.22151898734177217No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.21835443037974683No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT690.21835443037974683No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.21518987341772153No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT670.21202531645569622No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA660.2088607594936709No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA650.20569620253164558No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.20253164556962028No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT630.19936708860759494No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.19936708860759494No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCAGGTAGTTAGACCAGCAC630.19936708860759494No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT610.1930379746835443No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT600.18987341772151897No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT600.18987341772151897No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.18670886075949367No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.18670886075949367No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.18354430379746836No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.18037974683544303No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA550.1740506329113924No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG550.1740506329113924No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.16772151898734178No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA520.16455696202531644No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.16139240506329114No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.16139240506329114No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.15822784810126583No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT500.15822784810126583No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.15822784810126583No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC490.1550632911392405No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT480.1518987341772152No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT470.14873417721518986No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.14873417721518986No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCAGGTAGTTAGACCAGCAC460.14556962025316456No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT460.14556962025316456No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA460.14556962025316456No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT450.14240506329113925No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC440.13924050632911392No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.13924050632911392No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT430.1360759493670886No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.1360759493670886No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC430.1360759493670886No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT430.1360759493670886No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC430.1360759493670886No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.1329113924050633No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.1329113924050633No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT420.1329113924050633No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCAGGTAGTTAGACCAGCAC420.1329113924050633No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA420.1329113924050633No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT410.12974683544303797No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.12658227848101267No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA400.12658227848101267No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT400.12658227848101267No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT400.12658227848101267No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCAGGTAGTTAGACCAGCAC400.12658227848101267No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC390.12341772151898735No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT380.12025316455696203No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT380.12025316455696203No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA380.12025316455696203No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.11708860759493671No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA370.11708860759493671No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCAGGTAGTTAGACCAGCAC360.1139240506329114No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA360.1139240506329114No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA350.11075949367088608No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.11075949367088608No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT340.10759493670886076No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG340.10759493670886076No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA330.10443037974683544No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.10443037974683544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)