FastQCFastQC Report
Sun 21 May 2023
EGAF00007846676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37643
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12303.267539781632707No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11162.96469463113992No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9932.637940652976649No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8542.2686820922880746No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7632.026937279175411No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7461.981776160242276No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7021.8648885582976913No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6891.8303535849958825No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6881.827697048588051No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6401.7001833010121405No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5921.57266955343623No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5141.3654597136253752No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5101.3548335679940495No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4981.3229551311000718No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4861.291076694206094No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4661.2379459660494647No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4571.2140371383789814No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4501.1954413835241613No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4431.176845628669341No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4241.1263714369205429No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4011.0652710995404193No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3871.0280795898307786No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3791.0068272985681268No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3590.9536965704114975No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3360.8925962330313737No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3280.871343941768722No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3180.8447785776904072No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3030.8049305315729351No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2900.7703955582711262No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2870.7624259490476317No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2840.7544563398241373No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2840.7544563398241373No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2770.7358605849693169No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2600.690699466036182No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2600.690699466036182No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA2590.6880429296283506No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.6694471747735303No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2480.6588210291422044No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2440.6481948835108785No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2260.6003772281699121No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2190.5817814733150918No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA2140.5684987912759345No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.5392768907897882No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1910.5073984538958106No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1910.5073984538958106No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1810.48083308981749595No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1780.4728634805940015No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1720.45692426214701276No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1680.44629811651568685No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1640.435671970884361No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.42770236166086656No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.4144196796217092No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.4117631432138777No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.40910660680604627No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1530.4064500703982148No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.40113699758255184No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.40113699758255184No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1500.3984804611747204No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.39582392476688893No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.3851977791355631No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1430.3798847063199001No Hit
ATGGTATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1420.37722816991206864No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1400.3719150970964057No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1360.36128895146507983No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.36128895146507983No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1360.36128895146507983No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1310.34800626942592244No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1270.3373801237945966No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.32675397816327073No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1210.3214409053476078No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1140.3028451504927875No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1110.2948755412692931No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.29221900486146163No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1100.29221900486146163No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.2869059320457987No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1060.2815928592301357No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1030.27362325000664134No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.2629971043753155No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT960.25502749515182105No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA950.2523709587439896No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC950.2523709587439896No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA940.24971442233615812No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC940.24971442233615812No Hit
ATGGTATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT910.2417448131126637No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA890.23643174029700076No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA880.2337752038891693No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.2337752038891693No Hit
ATGGTATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT870.2311186674813378No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA860.22846213107350638No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC850.22580559466567487No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA820.2178359854421805No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA820.2178359854421805No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.2178359854421805No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT810.215179449034349No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.215179449034349No Hit
ATGGTATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT790.20986637621868606No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA780.2072098398108546No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC770.20455330340302313No Hit
ATGGTATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.20189676699519168No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA760.20189676699519168No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA720.1912706213638658No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.18595754854820284No Hit
ATGGTATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT690.1833010121403714No Hit
ATGGTATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC690.1833010121403714No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC680.18064447573253992No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA660.175331402916877No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA650.1726748665090455No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA630.16736179369338258No Hit
ATGGTATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.16736179369338258No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.16736179369338258No Hit
ATGGTATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT620.1647052572855511No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA620.1647052572855511No Hit
ATGGTATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT600.15939218446988818No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC600.15939218446988818No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.15939218446988818No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC590.1567356480620567No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.1567356480620567No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC580.15407911165422522No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA570.15142257524639374No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.14876603883856226No Hit
ATGGTATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC550.14610950243073081No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.14610950243073081No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.14610950243073081No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.14345296602289934No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.14079642961506786No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC520.1381398932072364No Hit
ATGGTATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT510.13548335679940493No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT500.13282682039157345No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.13282682039157345No Hit
ATGGTATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC500.13282682039157345No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT490.130170283983742No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.12485721116807906No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA460.1222006747602476No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGGAGGGTGCGAGCGTTA460.1222006747602476No Hit
ATGGTATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT460.1222006747602476No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA460.1222006747602476No Hit
ATGGTATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC460.1222006747602476No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.11954413835241612No Hit
ATGGTATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.11688760194458465No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.11688760194458465No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.11688760194458465No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG420.11157452912892171No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAACACGGAGGGTGCGAGCGTTA420.11157452912892171No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA420.11157452912892171No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA410.10891799272109025No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA410.10891799272109025No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA400.10626145631325878No Hit
ATGGTATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT400.10626145631325878No Hit
ATGGTATGACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.10626145631325878No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA390.1036049199054273No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA390.1036049199054273No Hit
ATGGTATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC390.1036049199054273No Hit
ATGGTATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT380.10094838349759584No Hit
ATGGTATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT380.10094838349759584No Hit
ATGGTATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT380.10094838349759584No Hit
ATGGTATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC380.10094838349759584No Hit
ATGGTATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC380.10094838349759584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)