FastQCFastQC Report
Sun 21 May 2023
EGAF00007846702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46935
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12722.7101310322786833No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10942.3308831362522637No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA9902.109300095877277No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8951.9068925109193564No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA8491.8088846276765742No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7491.5958240119313947No Hit
TACCGGTCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7221.538297645680196No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA7171.527644614892937No Hit
TACCGGTCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7041.4999467348460638No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7031.497816128688612No Hit
TACCGGTCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6951.4807712794289976No Hit
TACCGGTCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6841.4573346116970278No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6601.4062000639181849No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6501.3848940023436669No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6321.3465430915095344No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6161.3124533929903057No Hit
TACCGGTCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5641.2016618728028123No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5561.184617023543198No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5171.101523383402578No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5071.08021732182806No Hit
TACCGGTCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4440.945989133908597No Hit
TACCGGTCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4410.9395973154362416No Hit
TACCGGTCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4350.9268136784915307No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4340.9246830723340791No Hit
TACCGGTCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4330.9225524661766272No Hit
TACCGGTCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4220.8991157984446574No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4180.8905933738148504No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4130.8799403430275913No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3970.8458506445083626No Hit
TACCGGTCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3950.841589432193459No Hit
TACCGGTCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3680.7840630659422606No Hit
TACCGGTCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3650.7776712474699051No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3590.7648876105251944No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3590.7648876105251944No Hit
TACCGGTCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3530.7521039735804836No Hit
TACCGGTCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3430.7307979120059657No Hit
TACCGGTCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.7286673058485139No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3420.7286673058485139No Hit
TACCGGTCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3330.7094918504314478No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3310.7052306381165442No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3300.7031000319590924No Hit
TACCGGTCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.6263982102908278No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2930.6242676041333759No Hit
TACCGGTCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2740.5837860871417918No Hit
TACCGGTCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2740.5837860871417918No Hit
TACCGGTCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2660.5667412378821775No Hit
TACCGGTCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2570.5475657824651113No Hit
TACCGGTCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2570.5475657824651113No Hit
TACCGGTCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.541173963992756No Hit
TACCGGTCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.48151699158410566No Hit
TACCGGTCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2230.4751251731117503No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2230.4751251731117503No Hit
TACCGGTCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2110.4495578992223287No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2100.44742729306487694No Hit
TACCGGTCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.42612123149035896No Hit
TACCGGTCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2000.42612123149035896No Hit
TACCGGTCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1840.3920315329711303No Hit
TACCGGTCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1840.3920315329711303No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1810.3856397144987749No Hit
TACCGGTCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1760.37498668371151594No Hit
TACCGGTCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.37285607755406414No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1720.36646425908170877No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1700.36220304676680515No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1660.35368062213699797No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1640.3494194098220944No Hit
TACCGGTCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1630.3472888036646426No Hit
TACCGGTCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1610.34302759134973904No Hit
TACCGGTCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.3408969851922872No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1570.3345051667199318No Hit
TACCGGTCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1560.33237456056248005No Hit
TACCGGTCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.33024395440502824No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1500.31959092361776925No Hit
TACCGGTCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1500.31959092361776925No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.31746031746031744No Hit
TACCGGTCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1490.31746031746031744No Hit
TACCGGTCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1470.3131991051454139No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1450.30893789283051026No Hit
TACCGGTCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.3046766805156067No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1420.3025460743581549No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1380.2940236497283477No Hit
TACCGGTCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1370.2918930435708959No Hit
TACCGGTCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1360.28976243741344415No Hit
TACCGGTCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.2748481943112816No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.2663257696814744No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1200.2556727388942154No Hit
TACCGGTCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.2535421327367636No Hit
TACCGGTCGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1190.2535421327367636No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.2535421327367636No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.24715031426440823No Hit
TACCGGTCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.23862788963460105No Hit
TACCGGTCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1120.23862788963460105No Hit
TACCGGTCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.23436667731969746No Hit
TACCGGTCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1080.23010546500479384No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1060.22584425268989028No Hit
TACCGGTCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.22584425268989028No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1040.22158304037498666No Hit
TACCGGTCGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.2151912219026313No Hit
TACCGGTCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.2151912219026313No Hit
TACCGGTCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.21306061574517948No Hit
TACCGGTCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.20453819111537233No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG960.20453819111537233No Hit
TACCGGTCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC960.20453819111537233No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.20240758495792052No Hit
TACCGGTCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT910.19388516032811334No Hit
TACCGGTCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.18749334185575797No Hit
TACCGGTCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT880.18749334185575797No Hit
TACCGGTCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.18749334185575797No Hit
TACCGGTCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT870.18536273569830616No Hit
TACCGGTCGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC860.18323212954085438No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA840.1789709172259508No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA820.1747097049110472No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA810.1725790987535954No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA810.1725790987535954No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA790.1683178864386918No Hit
TACCGGTCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT790.1683178864386918No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA780.16618728028124002No Hit
TACCGGTCGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC770.16405667412378822No Hit
TACCGGTCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT770.16405667412378822No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.16405667412378822No Hit
TACCGGTCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC760.1619260679663364No Hit
TACCGGTCGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC750.15979546180888463No Hit
TACCGGTCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT750.15979546180888463No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA730.15553424949398104No Hit
TACCGGTCGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC730.15553424949398104No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA720.15340364333652923No Hit
TACCGGTCGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC720.15340364333652923No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA700.14914243102162564No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG690.14701182486417386No Hit
TACCGGTCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.14701182486417386No Hit
TACCGGTCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT680.14488121870672208No Hit
TACCGGTCGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.14275061254927027No Hit
TACCGGTCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.14275061254927027No Hit
TACCGGTCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT660.1406200063918185No Hit
TACCGGTCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.13848940023436668No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.13848940023436668No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.1363587940769149No Hit
TACCGGTCGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC640.1363587940769149No Hit
TACCGGTCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.1363587940769149No Hit
TACCGGTCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.1320975817620113No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA620.1320975817620113No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA620.1320975817620113No Hit
TACCGGTCGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT600.1278363694471077No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.1257057632896559No Hit
TACCGGTCGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.12357515713220411No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA580.12357515713220411No Hit
TACCGGTCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT570.12144455097475232No Hit
TACCGGTCGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC560.11931394481730052No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG560.11931394481730052No Hit
TACCGGTCGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT530.11292212634494514No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA520.11079152018749333No Hit
TACCGGTCGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT520.11079152018749333No Hit
TACCGGTCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.10653030787258974No Hit
TACCGGTCGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.10653030787258974No Hit
TACCGGTCGCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT490.10439970171513796No Hit
TACCGGTCGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.10226909555768617No Hit
TACCGGTCGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC470.10013848940023437No Hit
TACCGGTCGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.10013848940023437No Hit
TACCGGTCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.10013848940023437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)