FastQCFastQC Report
Sun 21 May 2023
EGAF00007846734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55977
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA27364.887721742858674No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA26914.807331582614288No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15602.786858888472051No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15332.738624792325419No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10561.886489093734927No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10091.8025260374796792No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9941.7757293173982174No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9621.7185629812244314No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9451.688193365132108No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8871.5845793808171214No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8671.5488504207085052No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8291.4809653965021348No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8261.4756060524858423No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7851.4023616842631794No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7771.388070100219733No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7291.3023205959590545No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7051.2594458438287155No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6821.218357539703807No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6321.1290351394322669No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6131.0950926273290815No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5951.062936563231327No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5921.0575772192150346No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5480.9789735069760795No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5380.9611090269217715No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5380.9611090269217715No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5270.9414580988620327No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5190.9271665148185861No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5180.9253800668131554No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5130.9164478267860013No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4740.8467763545742002No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4690.8378441145470461No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4580.8181931864873072No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4540.8110473944655842No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4170.7449488182646444No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4160.7431623702592136No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4110.7342301302320596No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3850.6877824820908587No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.685996034085428No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3770.6734908980474124No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3630.6484806259713811No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3610.6449077299605195No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3580.6395483859442271No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3460.6181110098790574No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3400.6073923218464726No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3320.5931007378030263No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3180.568090465726995No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3130.559158225699841No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3120.5573717776944102No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.550225985672687No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3070.5484395376672562No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3020.5395072976401022No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2920.5216428175857941No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2910.5198563695803633No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2910.5198563695803633No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2840.5073512335423478No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2710.4841274094717473No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.45375779337942374No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2470.4412526573414081No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.4233881772871001No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2360.4216017292816693No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2250.40195080122193044No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2180.3894456651839148No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.3769405291458992No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2080.3715811851296068No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.360862497097022No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.3537167050752988No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1970.35193025706986797No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1920.342998017042714No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1920.342998017042714No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1900.33942512103185235No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.33406577701556No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1860.3322793290101292No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1820.3251335369884059No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.32334708898297515No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1740.31084195294495953No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1720.3072690569340979No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1700.3036961609232363No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1640.2929774728906515No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.2876181288743591No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1530.27332654483091273No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.2590349607874663No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.2572485127820355No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.22509244868428105No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1170.20901441663540382No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1170.20901441663540382No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1170.20901441663540382No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.2000821766082498No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1110.19829572860281902No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1060.189363488575665No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.18757704057023422No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.1857905925648034No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1020.1822176965539418No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1000.1786448005430802No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC970.17328545652678778No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.16792611251049538No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA930.1661396645050646No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA870.15542097647247977No Hit
TAGCCAATCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT860.15363452846704895No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA850.15184808046161816No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT820.14648873644532576No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC800.14291584043446415No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.14112939242903336No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT790.14112939242903336No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC760.13577004841274096No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.13577004841274096No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA750.13398360040731014No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.13398360040731014No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.13398360040731014No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA740.13219715240187932No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC710.12683780838558695No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA700.12505136038015616No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.12326491237472532No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.11969201636386373No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG670.11969201636386373No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.11254622434214052No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA620.11075977633670973No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA620.11075977633670973No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.10897332833127892No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT610.10897332833127892No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.10718688032584811No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT590.10540043232041732No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.10540043232041732No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCAAGCGTTA590.10540043232041732No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.10361398431498652No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA580.10361398431498652No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT580.10361398431498652No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.1018275363095557No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.1000410883041249No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.1000410883041249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)