FastQCFastQC Report
Sun 21 May 2023
EGAF00007846758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46552
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15403.30812854442344No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG12322.6465028355387523No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12042.5863550438219627No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10182.1868018559890015No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8361.7958412098298677No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8041.7271008764392508No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8001.7185083347654235No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7861.6884344389070285No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7641.6411754597009796No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7201.5466575012888812No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6831.4671764908059806No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6771.4542876782952396No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6591.4156212407630178No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6351.364065990720055No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6221.3361402302801169No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6171.3253995531878329No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5751.2351778656126482No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5561.1943632926619694No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5311.1406599072005499No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5171.110586011342155No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4691.0074755112562295No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4580.983846021653205No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4380.9408833132840694No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4360.9365870424471558No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4140.8893280632411068No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4060.8721429798934526No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3950.8485134902904279No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3930.8442172194535142No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3710.7969582402474652No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3630.779773156899811No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3350.7196253651830211No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.7131809589276508No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3250.6981440109984534No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3070.6594775734662314No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.6465887609554907No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2960.6358480838632067No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2940.6315518130262932No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2930.6294036776078364No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2920.6272555421893796No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2920.6272555421893796No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2880.6186630005155525No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2870.6165148650970957No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2830.6079223234232686No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.5971816463309847No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2770.595033510912528No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.584292833820244No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2680.5757002921464169No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2550.5477745317064787No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.5413301254511085No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.5413301254511085No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.5134043650111703No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.5134043650111703No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2390.5134043650111703No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2220.47688606289740504No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2080.4468121670390101No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.4231826774359856No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.4017013232514178No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1850.39740505241450425No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.39310878157759066No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.39096064615913384No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.3866643753222203No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1740.37377556281147967No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.36088675030073897No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1640.35229420862691185No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1640.35229420862691185No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1610.3458498023715415No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1610.3458498023715415No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1590.3415535315346279No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1580.33940539611617115No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1570.3372572606977144No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.3351091252792576No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.3329609898608008No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1550.3329609898608008No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1480.31792404193160334No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1460.3136277710946898No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1450.311479635676233No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.311479635676233No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.3028870940024059No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1400.3007389585839491No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1360.292146416910122No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.2857020106547517No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1330.2857020106547517No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1330.2857020106547517No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1310.2814057398178381No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.27925760439938135No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1290.2771094689809246No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1230.2642206564701839No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1220.2620725210517271No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2599243856332703No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.2599243856332703No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1190.2556281147963568No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1170.2513318439594432No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.24273930228561608No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.2405911668671593No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1120.2405911668671593No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1080.2319986251933322No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.2191098126825915No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1020.2191098126825915No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA970.2083691355903076No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA960.20622100017185083No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.20407286475339403No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT950.20407286475339403No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA930.19977659391648048No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC930.19977659391648048No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT900.19333218766111016No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA890.1911840522426534No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC880.1890359168241966No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.18688778140573983No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC870.18688778140573983No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA870.18688778140573983No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA860.18473964598728304No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA840.18044337515036948No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA840.18044337515036948No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA830.1782952397319127No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA820.17614710431345593No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC810.17399896889499913No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA810.17399896889499913No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA800.17185083347654237No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA800.17185083347654237No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA800.17185083347654237No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA770.16540642722117202No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.16540642722117202No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.16325829180271523No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC740.15896202096580167No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC730.1568138855473449No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.15251761471043135No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT710.15251761471043135No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG710.15251761471043135No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC700.15036947929197456No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.14392507303660423No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT670.14392507303660423No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.14177693761814747No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC650.13962880219969068No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG640.13748066678123388No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC640.13748066678123388No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.13533253136277712No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC620.13318439594432033No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC620.13318439594432033No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.13103626052586356No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG600.12888812510740677No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT600.12888812510740677No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.12673998968895No Hit
TAGCCAATCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.12459185427049321No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT580.12459185427049321No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA580.12459185427049321No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT580.12459185427049321No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC570.12244371885203643No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC560.12029558343357966No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.12029558343357966No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA560.12029558343357966No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC550.11814744801512288No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT520.11170304175975253No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.11170304175975253No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.10955490634129575No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC500.10740677092283897No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA490.10525863550438219No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.10311050008592541No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA480.10311050008592541No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC480.10311050008592541No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC480.10311050008592541No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT480.10311050008592541No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.10311050008592541No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC470.10096236466746863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)