FastQCFastQC Report
Sun 21 May 2023
EGAF00007846784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58615
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG16812.867866587051096No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16092.745031135374904No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG14452.4652392732235775No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12412.1172054934743665No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10351.7657596178452615No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9841.6787511729079587No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9671.6497483579288579No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9521.624157638829651No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9481.6173334470698626No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9301.5866245841508146No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9071.547385481532031No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9051.543973385652137No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8181.395547214876738No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8131.3870169751770025No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7341.2522391879211807No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7091.2095879894225028No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6821.1635246950439306No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6601.1259916403650942No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6451.1004009212658876No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6441.0986948733259405No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6371.0867525377463108No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6341.0816343939264692No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6241.0645739145269981No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5871.001450140748955No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5450.9297961272711763No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5350.9127356478717051No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5350.9127356478717051No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4950.8444937302738207No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4870.8308453467542437No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4810.8206090591145611No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4730.8069606755949843No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4660.7950183400153543No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4490.7660155250362536No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4440.7574852853365179No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.7540731894566237No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3970.6773010321590037No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3970.6773010321590037No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3960.6755949842190565No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3900.6653586965793739No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3830.6534163609997441No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3760.6414740254201143No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3730.636355881600273No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3710.6329437857203788No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3640.6210014501407489No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3480.5937046831015952No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3350.5715260598822827No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3260.5561716284227587No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3260.5561716284227587No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3220.5493474366629703No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3080.5254627655037106No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3060.5220506696238164No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3060.5220506696238164No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3000.5118143819841338No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2730.4657510876055617No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.4589268958457733No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2670.45551479996587907No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2600.4435724643862492No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2380.40603940970741276No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2380.40603940970741276No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2340.3992152179476243No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2320.3958031220677301No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2320.3958031220677301No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.39239102618783583No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2270.3872728823679945No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2180.37191845090847053No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2130.36338821120873493No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2020.34462168386931674No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.3412095879894225No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.3412095879894225No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1990.3395035400494754No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1970.33609144416958114No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1880.32073701271005717No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1820.31050072507037446No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.30879467713042735No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1780.303676533310586No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1670.2849100059711678No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1650.28149791009127356No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1630.27808581421137935No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1620.27637976627143224No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1600.27296767039153796No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1600.27296767039153796No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.27296767039153796No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.27296767039153796No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.2695555745116438No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.26784952657169664No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1550.2644374306918025No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1540.2627313827518553No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1520.2593192868719611No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1460.24908299923227842No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1460.24908299923227842No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.24567090335238423No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.23714066365264863No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1390.23714066365264863No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1380.23543461571270152No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.2337285677727544No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1370.2337285677727544No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1360.2320225198328073No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1330.22690437601296598No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1300.2217862321931246No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1300.2217862321931246No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1280.2183741363132304No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1240.21154994455344195No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1220.2081378486735477No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1160.19790156103386505No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1150.19619551309391792No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1130.19278341721402373No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1120.1910773692740766No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.18766527339418237No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1100.18766527339418237No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1080.18425317751428813No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1010.17231084193465837No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1010.17231084193465837No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1010.17231084193465837No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA990.16889874605476413No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC990.16889874605476413No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC980.16719269811481702No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC970.1654866501748699No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.1637806022349228No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA940.16036850635502858No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC920.15695641047513434No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA910.15525036253518723No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.15525036253518723No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA900.15354431459524012No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA900.15354431459524012No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC890.151838266655293No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT870.1484261707753988No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.14672012283545166No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA860.14672012283545166No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.14501407489550458No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA850.14501407489550458No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA840.14330802695555744No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC820.13989593107566323No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC820.13989593107566323No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.1347777872558219No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.13307173931587477No Hit
TAGCCAATCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT780.13307173931587477No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG780.13307173931587477No Hit
TAGCCAATCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT760.12965964343598055No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT760.12965964343598055No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.12795359549603344No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.12624754755608633No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT720.12283545167619211No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT720.12283545167619211No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT710.12112940373624498No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.11942335579629788No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC700.11942335579629788No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.11771730785635076No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT690.11771730785635076No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA680.11601125991640365No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC660.11259916403650944No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.11259916403650944No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.1108931160965623No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC650.1108931160965623No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.10748102021666808No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC620.10577497227672097No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA620.10577497227672097No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA620.10577497227672097No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA620.10577497227672097No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.10577497227672097No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA600.10236287639682676No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.10236287639682676No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA600.10236287639682676No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.10065682845687965No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.10065682845687965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)