FastQCFastQC Report
Sun 21 May 2023
EGAF00007846806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54068
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA11292.0881112672930384No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11052.043722719538359No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA10872.01043130872235No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9891.8291780720574091No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9831.8180809351187395No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8771.6220315158689058No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8321.5388029888288821No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8051.488865872604868No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8031.4851668269586447No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7871.4555744617888584No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7791.4407782792039654No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7041.3020640674705926No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6851.2669231338314715No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6531.2077384034918992No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6491.2003403121994525No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6491.2003403121994525No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6421.1873936524376711No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5981.106014648220759No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5931.096767034105201No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5901.091218465635866No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5491.0153880298882887No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5461.0098394614189539No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5380.9950432788340609No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5300.9802470962491677No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5060.9358585484944884No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4940.9136642746171488No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4640.8581785899237996No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4570.8452319301620181No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4540.8396833616926833No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4480.8285862247540134No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4380.8100909965228972No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4360.8063919508766738No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4270.789746245468669No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3900.7213139010135385No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.7213139010135385No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3840.7102167640748687No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3720.688022490197529No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3580.6621291706739661No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3520.6510320337352963No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3510.6491825109121847No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3420.6325368055041799No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC3380.6251387142117334No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3320.6140415772730635No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3190.5899977805726123No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3150.5825996892801657No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.5752015979877192No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3060.5659539838721609No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2980.5511578012872679No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2970.5493082784641562No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2960.5474587556410446No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2900.5363616187023748No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2870.5308130502330399No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2870.5308130502330399No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.5234149589405933No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2720.5030702078863654No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2710.5012206850632537No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2620.484574979655249No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2520.4660797514241326No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2440.4512835688392395No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.44943404601612785No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2410.4457350003699046No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.43833690907745804No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.41799215802323003No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2180.4031959754383369No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2150.397647406969002No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2140.39579788414589034No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2090.38655027003033215No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2060.3810017015609973No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC2050.3791521787378856No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2040.37730265591477397No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1950.36065695050676927No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1940.35880742768365764No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1820.33661315380631796No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.3236664940445365No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1740.3218169712214249No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1740.3218169712214249No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.30887031145964344No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1650.30517126581342013No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1600.29592365169786194No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1590.2940741288747503No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1570.290375083228527No Hit
TAGCCAATCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1570.290375083228527No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.28852556040541544No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.28852556040541544No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1550.28667603758230376No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1530.28297699193608045No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1500.27742842346674557No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2737293778205223No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1440.2663312865280758No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1410.2607827180587408No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1350.24968558112007105No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.24783605829695937No Hit
TAGCCAATCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1330.24598653547384777No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1320.2441370126507361No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1270.23488939853517793No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1260.2330398757120663No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1250.23119035288895465No Hit
TAGCCAATCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1240.22934083006584302No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.22749130724273137No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1230.22749130724273137No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.2200932159502848No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1180.21824369312717318No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.2145446474809499No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1160.2145446474809499No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.2126951246578383No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.21084560183472661No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1130.20899607901161502No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1120.20714655618850336No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1100.2034475105422801No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.20159798771916845No Hit
TAGCCAATCCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC1040.19235037360361026No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1030.19050085078049864No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1020.18865132795738698No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.18495228231116373No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.18495228231116373No Hit
TAGCCAATCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC990.18310275948805207No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.17570466819560554No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA930.17200562254938226No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT920.17015609972627063No Hit
TAGCCAATCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC900.16645705408004735No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA890.1646075312569357No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA860.1590589627876008No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA850.15720943996448916No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA850.15720943996448916No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA840.15535991714137753No Hit
TAGCCAATCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT830.15351039431826588No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA820.15166087149515425No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1498113486720426No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA810.1498113486720426No Hit
TAGCCAATCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC800.14796182584893097No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA790.14611230302581935No Hit
TAGCCAATCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC770.14241325737959606No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA770.14241325737959606No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT760.14056373455648444No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC750.13871421173337278No Hit
TAGCCAATCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT740.13686468891026116No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT740.13686468891026116No Hit
TAGCCAATCCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC740.13686468891026116No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA730.1350151660871495No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.1350151660871495No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT720.1331656432640379No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA710.13131612044092625No Hit
TAGCCAATCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.13131612044092625No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT700.12946659761781462No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA700.12946659761781462No Hit
TAGCCAATCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.12761707479470297No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC680.12576755197159134No Hit
TAGCCAATCCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC660.12206850632536805No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA650.1202189835022564No Hit
TAGCCAATCCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC640.11836946067914478No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.11651993785603315No Hit
TAGCCAATCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.11467041503292151No Hit
TAGCCAATCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT620.11467041503292151No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA620.11467041503292151No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC610.11282089220980987No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA610.11282089220980987No Hit
TAGCCAATCCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.11282089220980987No Hit
TAGCCAATCCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT600.11097136938669823No Hit
TAGCCAATCCCGGACTACACGGGTATCTAATCCTGTTCGATACCCGCACC600.11097136938669823No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG590.10912184656358659No Hit
TAGCCAATCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT580.10727232374047495No Hit
TAGCCAATCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.10727232374047495No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA580.10727232374047495No Hit
TAGCCAATCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.10727232374047495No Hit
TAGCCAATCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.10542280091736331No Hit
TAGCCAATCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT570.10542280091736331No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA560.10357327809425168No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA560.10357327809425168No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.10357327809425168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)