FastQCFastQC Report
Sun 21 May 2023
EGAF00007846850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42503
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16323.8397289603086837No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10172.3927722748982423No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8632.030444909771075No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7441.7504646730819No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7031.6540008940545372No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6771.5928287415006002No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6681.5716537656165448No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6291.4798955367856386No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6261.4728372114909536No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6091.4328400348210715No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5831.371667882267134No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5781.3599040067759922No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5751.3528456814813072No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5501.2940263040255982No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4971.169329223819495No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4771.122273721854928No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4541.0681598945956756No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4531.0658071194974472No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4511.0611015693009904No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4471.051690468908077No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4341.0211043926311085No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.985812766157683No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4170.9811072159612263No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4130.9716961155683127No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3910.9199350634072888No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3750.8822906618356351No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3700.8705267863444933No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3680.8658212361480366No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3550.8352351598710679No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3540.8328823847728395No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3370.7928852081029574No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.7858268828082724No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3220.757593581629532No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3120.7340658306472484No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3040.7152436298614215No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2970.698774204173823No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2860.672893678093311No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2850.6705409029950827No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2840.6681881278968543No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2820.6634825777003976No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2780.6540714773074842No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2630.6187798508340588No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2630.6187798508340588No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2470.581135449262405No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2430.5717243488694915No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2400.5646660235748064No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.5599604733783498No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.5576076982801214No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.5317271721996094No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2240.5270216220031527No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2080.48937722043149895No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2040.47996612003858546No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2010.4729077947439004No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2000.4705550196456721No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.4611439192527586No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1910.44938004376161683No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.44938004376161683No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1900.44702726866338843No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1830.4305578429757899No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1820.4282050678775616No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1770.41644119238641975No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1720.40467731689527797No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1680.3952662165023646No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1660.39056066630590786No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.37409124061830934No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1520.35762181493071077No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.35526903983248237No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1510.35526903983248237No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1510.35526903983248237No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1500.352916264734254No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.352916264734254No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.3293885137519705No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.3270357386537421No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.32468296355551374No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1380.32468296355551374No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1380.32468296355551374No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1340.3152718631626003No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1280.30115521257323014No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1270.29880243747500174No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.29880243747500174No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1250.29409688727854505No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.29409688727854505No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.2799802366891749No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.27762746159094653No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.2729219113944898No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1150.27056913629626145No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1140.2682163611980331No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1140.2682163611980331No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1110.261158035903348No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1090.2564524857068913No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.2517469355104346No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.2493941604122062No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.24468861021574947No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1040.24468861021574947No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.24468861021574947No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.24233583511752113No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC920.21645530903700916No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.21174975884055242No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT890.20939698374232407No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT880.20704420864409573No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA880.20704420864409573No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC880.20704420864409573No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTA860.20233865844763899No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.19528033315295393No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.19292755805472556No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG810.19057478295649719No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC800.18822200785826884No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA800.18822200785826884No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC800.18822200785826884No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT800.18822200785826884No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA780.1835164576618121No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT710.16704703197421358No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.16704703197421358No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT700.16469425687598524No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA700.16469425687598524No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.15763593158130015No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC670.15763593158130015No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.15763593158130015No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA650.15293038138484344No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC650.15293038138484344No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT650.15293038138484344No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.15057760628661507No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.15057760628661507No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT630.1482248311883867No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT630.1482248311883867No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.14351928099192998No Hit
TAGGTGTACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT610.14351928099192998No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA610.14351928099192998No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT600.1411665058937016No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA590.13881373079547327No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC580.1364609556972449No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT570.13410818059901655No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC570.13410818059901655No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.13410818059901655No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC550.1294026304025598No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC550.1294026304025598No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTA540.12704985530433147No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT530.1246970802061031No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT530.1246970802061031No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG520.12234430510787474No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT520.12234430510787474No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA510.11999153000964637No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.11999153000964637No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.11763875491141802No Hit
TAGGTGTACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT500.11763875491141802No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA500.11763875491141802No Hit
TAGGTGTACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT490.11528597981318965No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA490.11528597981318965No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC480.11293320471496131No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT480.11293320471496131No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA480.11293320471496131No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC470.11058042961673294No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA470.11058042961673294No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.11058042961673294No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.11058042961673294No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT460.10822765451850458No Hit
TAGGTGTACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT460.10822765451850458No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC450.10587487942027621No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA450.10587487942027621No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC450.10587487942027621No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC450.10587487942027621No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA440.10352210432204786No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT440.10352210432204786No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC440.10352210432204786No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA430.10116932922381949No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.10116932922381949No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC430.10116932922381949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)