FastQCFastQC Report
Sun 21 May 2023
EGAF00007846851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42503
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15653.6820930287273836No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11172.628049784721078No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8471.9928005081994211No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7941.868103427993318No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7881.853986777403948No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7151.6822341952332776No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7051.658706444250994No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6631.559889890125403No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6421.5104816130626075No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6321.4869538620803238No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6081.430487259722843No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5921.3928428581511894No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5561.3081429546149683No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5111.2022680751946921No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5081.195209749900007No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4981.1716819989177234No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4851.1410959226407547No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4501.0587487942027622No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4441.044632143613392No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4401.0352210432204785No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4341.0211043926311085No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3970.934051713996659No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.9246406136037456No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3920.9222878385055172No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3860.908171187916147No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3850.9058184128179189No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.9034656377196905No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3620.8517045855586665No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3450.8117074088887843No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3410.802296308495871No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.802296308495871No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3340.7858268828082724No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3310.7787685575135873No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3220.757593581629532No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3120.7340658306472484No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2910.6846575535844529No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2910.6846575535844529No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2850.6705409029950827No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2720.639954826718114No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2720.639954826718114No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2700.6352492765216573No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2570.6046632002446886No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2540.5976048749500035No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2470.581135449262405No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.581135449262405No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2450.5764298990659482No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2300.5411382725925229No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT2270.5340799472978378No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2240.5270216220031527No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2180.5129049714137826No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2050.48231889513681386No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2050.48231889513681386No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.4752605698421288No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2010.4729077947439004No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1970.463496694350987No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.4611439192527586No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1850.43526339317224666No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1840.4329106180740183No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1840.4329106180740183No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1840.4329106180740183No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1830.4305578429757899No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1760.41408841728819146No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1700.3999717666988213No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1660.39056066630590786No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.3764440157165377No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1570.3693856904218526No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1570.3693856904218526No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1540.36232736512716746No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1470.34585793943956894No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.34115238924311225No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.3152718631626003No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.3152718631626003No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.3152718631626003No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1340.3152718631626003No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1280.30115521257323014No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1270.29880243747500174No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.29880243747500174No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1240.29174411218031665No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1240.29174411218031665No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.2893913370820883No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.2893913370820883No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1190.2799802366891749No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.27762746159094653No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.27762746159094653No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.2729219113944898No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1140.2682163611980331No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1140.2682163611980331No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.2658635860998047No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1100.2588052608051196No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.2588052608051196No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1100.2588052608051196No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.2564524857068913No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1080.25409971060866293No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1080.25409971060866293No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1060.2493941604122062No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1060.2493941604122062No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT1060.2493941604122062No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.24468861021574947No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1040.24468861021574947No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1030.24233583511752113No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTA970.22821918452815096No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.22821918452815096No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC930.2188080841352375No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.20939698374232407No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC880.20704420864409573No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.19998588334941064No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT850.19998588334941064No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG820.19292755805472556No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.18586923276004047No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC780.1835164576618121No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1835164576618121No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.17881090746535538No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.17881090746535538No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.16469425687598524No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA690.16234148177775687No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA660.15528315648307178No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.15528315648307178No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.15293038138484344No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC650.15293038138484344No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT650.15293038138484344No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA640.15057760628661507No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT640.15057760628661507No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA630.1482248311883867No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC620.14587205609015833No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.14587205609015833No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.14587205609015833No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.1411665058937016No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC600.1411665058937016No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT590.13881373079547327No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC590.13881373079547327No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT570.13410818059901655No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA560.13175540550078818No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.13175540550078818No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.1294026304025598No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT540.12704985530433147No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC540.12704985530433147No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTA540.12704985530433147No Hit
TAGGTGTACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT530.1246970802061031No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC530.1246970802061031No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.1246970802061031No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT520.12234430510787474No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.12234430510787474No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC510.11999153000964637No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.11999153000964637No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT510.11999153000964637No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.11763875491141802No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCC490.11528597981318965No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT490.11528597981318965No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA470.11058042961673294No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA470.11058042961673294No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.11058042961673294No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT460.10822765451850458No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT460.10822765451850458No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC460.10822765451850458No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC460.10822765451850458No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT450.10587487942027621No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.10587487942027621No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT450.10587487942027621No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA450.10587487942027621No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCT440.10352210432204786No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA440.10352210432204786No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT440.10352210432204786No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.10352210432204786No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA430.10116932922381949No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.10116932922381949No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACT430.10116932922381949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)