FastQCFastQC Report
Sun 21 May 2023
EGAF00007846906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62162
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13512.1733534957047715No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13032.096135902963225No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12622.0301792091631543No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA12401.9947878124899459No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11881.9111354203532704No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA11411.8355265274605064No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9881.5893954505968275No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9431.517003957401628No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9141.4703516617869439No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9011.449438563752775No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8911.4333515652649529No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8631.388307969499051No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8601.3834818699527043No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8481.3641774717673176No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8371.3464817734307133No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8261.328786075094109No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8011.2885685788745536No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7791.2531771822013449No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7511.2081335864354428No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6561.0553071008011325No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6521.0488723014060035No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6281.0102635050352307No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6050.9732634085132397No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5690.9153502139570798No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5680.9137415141082976No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5670.9121328142595154No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5120.8236543225764937No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4960.7979151249959783No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4930.7930890254496316No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4820.7753933271130273No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4680.7528715292300763No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4640.7464367298349474No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4500.7239149319519964No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4460.7174801325568675No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4260.6853061355812232No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3990.6418712396641035No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3980.6402625398153213No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3820.6145233422348059No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3520.5662623467713395No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3490.5614362472249927No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3460.5566101476786461No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC3460.5566101476786461No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA3440.5533927479810817No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3390.5453492487371706No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA3320.5340883497956951No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.5260448505517841No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3130.5035230526688331No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.5003056529712686No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3100.49869695312248646No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3080.495479553424922No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2980.47939255493709987No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2950.4745664553907532No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.47134905569318875No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2910.46813165599562434No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA2720.43756635886876233No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2720.43756635886876233No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2560.41182716128824687No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2520.405392361893118No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.40217496219555354No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2500.40217496219555354No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2470.3973488626492069No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2410.38769666355651367No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2310.3716096650686915No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2270.3651748656735626No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2240.36034876612721595No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2230.35874006627843374No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2200.35391396673208714No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.35230526688330494No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2180.35069656703452273No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2130.3426530677906116No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.3426530677906116No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2100.337826968244265No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC2050.3297834690003539No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.3281747691515717No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2030.3265660693027895No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2020.32495736945400727No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2020.32495736945400727No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1980.3185225700588784No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1950.3136964705125318No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1940.31208777066374954No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1930.31047907081496734No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1890.3040442714198385No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1890.3040442714198385No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1850.2976094720247096No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.28473987323445193No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.28152247353688753No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1730.27830507383932307No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1630.2622180753515009No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1630.2622180753515009No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1630.2622180753515009No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1570.25256587625880766No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1510.24291367716611437No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.24130497731733214No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.2364788777709855No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1450.23326147807342107No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1450.23326147807342107No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1400.22521797882950997No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1350.21717447958559893No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1340.21556577973681673No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1300.20913098034168787No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1300.20913098034168787No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1280.20591358064412343No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1260.202696180946559No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.202696180946559No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1240.19947878124899457No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.19787008140021234No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1200.1930439818538657No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1170.18821788230751907No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1140.1833917827611724No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1100.17695698336604357No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA1080.17373958366847914No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.17373958366847914No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1010.16247868472700364No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.16247868472700364No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA1000.1608699848782214No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA980.157652585180657No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG950.15282648563431034No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA940.15121778578552814No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC940.15121778578552814No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC940.15121778578552814No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT930.1496090859367459No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT920.1480003860879637No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC920.1480003860879637No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT910.14639168623918147No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA910.14639168623918147No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC900.14478298639039927No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC890.14317428654161707No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT890.14317428654161707No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.14156558669283487No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA880.14156558669283487No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT870.13995688684405264No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC860.13834818699527043No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG860.13834818699527043No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT850.1367394871464882No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA840.135130787297706No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC820.13191338760014157No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT820.13191338760014157No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.13030468775135934No Hit
TAGGTGTACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT810.13030468775135934No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT810.13030468775135934No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT800.12869598790257714No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.1270872880537949No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCTAGCT790.1270872880537949No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA790.1270872880537949No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA780.12547858820501273No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT780.12547858820501273No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.1238698883562305No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT730.11743508896110164No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.11743508896110164No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA720.11582638911231942No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT710.1142176892635372No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA710.1142176892635372No Hit
TAGGTGTACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT710.1142176892635372No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA710.1142176892635372No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC710.1142176892635372No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA700.11260898941475499No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT690.11100028956597277No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.11100028956597277No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA690.11100028956597277No Hit
TAGGTGTACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.10778288986840837No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA660.10617419001962615No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.10456549017084393No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC640.10295679032206172No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.1013480904732795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)