FastQCFastQC Report
Sun 21 May 2023
EGAF00007846907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62162
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13892.2344840899584955No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA13172.118657700846176No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13002.091309803416878No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA12572.0221357099192434No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG11941.9207876194459639No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11651.8741353238312795No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11611.8677005244361506No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10201.6408738457578584No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9981.6054824490846498No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9601.5443518548309256No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8961.4413950645088638No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8741.4060036678356553No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8321.3384382741868022No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8241.3255686753965445No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8101.3030468775135935No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8091.3014381776648112No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7801.2547858820501272No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7431.1952639876451852No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6821.09713329686947No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6551.0536984009523502No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6241.0038287056401018No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6170.9925678066986262No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5770.9282198127473376No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5750.9250024130497732No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5410.8703066181911779No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5390.8670892184936135No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5330.8574370194009202No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5330.8574370194009202No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5230.8413500209130981No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5230.8413500209130981No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5080.8172195231813648No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4760.765741128020334No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4740.7625237283227696No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4680.7528715292300763No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4470.7190888324056497No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC4410.7094366333129565No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4290.6901322351275699No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4150.6676104372446189No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC4060.653132138605579No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA4020.64669733921045No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3810.6129146423860237No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA3770.6064798429908947No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3770.6064798429908947No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3560.5726971461664683No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3470.5582188475274283No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3150.5067404523663975No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3140.5051317525176153No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3060.4922621537273576No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3030.48743605418101094No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2960.4761751552395354No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2940.472957755541971No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.45043595765901995No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2740.4407837585663267No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2710.43595765901998007No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.4263054599272868No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2630.42308806022972234No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2610.41987066053215794No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2560.41182716128824687No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2500.40217496219555354No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2470.3973488626492069No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2460.3957401628004247No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA2420.3893053634052958No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2410.38769666355651367No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2360.37965316431260254No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2350.37804446446382034No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2260.3635661658247804No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2230.35874006627843374No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2090.33621826839548274No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC2070.33300086869791834No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2060.33139216884913614No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.3281747691515717No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2020.32495736945400727No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1990.32013126990766066No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1970.3169138702100962No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1950.3136964705125318No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1930.31047907081496734No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1920.30887037096618514No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.30243557157105627No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1820.29278337247836295No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1800.28956597278079854No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1800.28956597278079854No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1800.28956597278079854No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1730.27830507383932307No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1700.2734789742929764No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1690.2718702744441942No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1670.2686528747466298No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1610.2590006756539365No Hit
TAGGTGTACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1600.25739197580515427No Hit
TAGGTGTACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1570.25256587625880766No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1530.2461310768636788No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1530.2461310768636788No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1500.24130497731733214No Hit
TAGGTGTACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1400.22521797882950997No Hit
TAGGTGTACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.22200057913194554No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.21556577973681673No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1340.21556577973681673No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.2139570798880345No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1290.20752228049290564No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1280.20591358064412343No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1270.2043048807953412No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.19787008140021234No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA1210.1946526817026479No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.1946526817026479No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1210.1946526817026479No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1200.1930439818538657No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1160.18660918245873684No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.1833917827611724No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1130.1817830829123902No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1130.1817830829123902No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.17856568321482577No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1100.17695698336604357No Hit
TAGGTGTACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1070.17213088381969693No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1040.16730478427335027No Hit
TAGGTGTACCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1030.16569608442456807No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA1020.16408738457578584No Hit
TAGGTGTACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.16247868472700364No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.1608699848782214No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.1608699848782214No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC980.157652585180657No Hit
TAGGTGTACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT970.15604388533187477No Hit
TAGGTGTACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC960.15443518548309257No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT940.15121778578552814No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT900.14478298639039927No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT900.14478298639039927No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT880.14156558669283487No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA850.1367394871464882No Hit
TAGGTGTACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC850.1367394871464882No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG830.13352208744892377No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.13191338760014157No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT810.13030468775135934No Hit
TAGGTGTACCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC810.13030468775135934No Hit
TAGGTGTACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT810.13030468775135934No Hit
TAGGTGTACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT810.13030468775135934No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC800.12869598790257714No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA800.12869598790257714No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.12869598790257714No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.1238698883562305No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG760.12226118850744828No Hit
TAGGTGTACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC750.12065248865866607No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA750.12065248865866607No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA740.11904378880988385No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA730.11743508896110164No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.11743508896110164No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.11582638911231942No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT720.11582638911231942No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT710.1142176892635372No Hit
TAGGTGTACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.1142176892635372No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG710.1142176892635372No Hit
TAGGTGTACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC700.11260898941475499No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA700.11260898941475499No Hit
TAGGTGTACCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC700.11260898941475499No Hit
TAGGTGTACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT690.11100028956597277No Hit
TAGGTGTACCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.11100028956597277No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.10778288986840837No Hit
TAGGTGTACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT660.10617419001962615No Hit
TAGGTGTACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT660.10617419001962615No Hit
TAGGTGTACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT660.10617419001962615No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA650.10456549017084393No Hit
TAGGTGTACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC650.10456549017084393No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG630.1013480904732795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)