FastQCFastQC Report
Sun 21 May 2023
EGAF00007846990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007846990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32706
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA20266.194582033877576No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA19445.943863511282334No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9602.935241240139424No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9242.8251696936341957No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7492.2900996759004464No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7222.2075460160215252No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6702.0485537821806394No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6121.8712162905888827No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5851.7886626307099616No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4861.4859658778205835No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4791.4645630771112335No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4441.3575490735644835No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4291.311685929187305No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3491.0670824925090199No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3481.0640249495505412No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3351.0242768910903197No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3281.0028740903809699No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3120.9539534030453128No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3070.93866568825292No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2870.8775148290833485No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2860.87445728612487No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2850.8713997431663913No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2840.868342200207913No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.8591695713324773No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2790.8530544854155201No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2750.8408243135816059No Hit
TCTAAGTTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.7643857396196416No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.7643857396196416No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2360.7215801382009417No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2340.7154650522839846No Hit
TCTAAGTTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.7154650522839846No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2330.712407509325506No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2240.684889622699199No Hit
TCTAAGTTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2140.6543141931144133No Hit
TCTAAGTTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.6359689353635418No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1980.6053935057787562No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1960.599278419861799No Hit
TCTAAGTTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.5656454473185348No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1740.5320124747752706No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1700.5197823029413563No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1680.5136672170243992No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1630.49837950223200633No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1590.4861493303980921No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1580.4830917874396135No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.4647465296887422No Hit
TCTAAGTTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.4555739008133064No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.4555739008133064No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1440.4402861860209136No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1370.41888338531156366No Hit
TCTAAGTTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1350.41276829939460646No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1340.4097107564361279No Hit
TCTAAGTTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.4066532134776493No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1310.40053812756069224No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.39748058460221364No Hit
TCTAAGTTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1290.3944230416437351No Hit
TCTAAGTTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1280.3913654986852565No Hit
TCTAAGTTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1280.3913654986852565No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.3821928698098208No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1230.37607778389286367No Hit
TCTAAGTTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.3730202409343851No Hit
TCTAAGTTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.3699626979759066No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1190.36384761205894944No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1190.36384761205894944No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.3577325261419923No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.3577325261419923No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1060.32409955359872805No Hit
TCTAAGTTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.32104201064024945No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1030.31492692472329237No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA960.2935241240139424No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA960.2935241240139424No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.29046658105546386No Hit
TCTAAGTTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT930.28435149513850666No Hit
TCTAAGTTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT920.2812939521800281No Hit
TCTAAGTTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT920.2812939521800281No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.27517886626307103No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC870.2660062373876353No Hit
TCTAAGTTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT850.25989115147067815No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC840.2568336085121996No Hit
TCTAAGTTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT820.25071852259524247No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG800.24460343667828532No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.24154589371980675No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA770.23543080780284964No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.2323732648443711No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA760.2323732648443711No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA750.2293157218858925No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA730.22320063596893538No Hit
TCTAAGTTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT730.22320063596893538No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC710.21708555005197824No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA690.21097046413502107No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.21097046413502107No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.21097046413502107No Hit
TCTAAGTTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT680.20791292117654253No Hit
TCTAAGTTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.19874029230110682No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC640.19568274934262825No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC620.18956766342567113No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA610.18651012046719256No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA610.18651012046719256No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.18039503455023542No Hit
TCTAAGTTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT580.17733749159175688No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC580.17733749159175688No Hit
TCTAAGTTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT570.1742799486332783No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.1742799486332783No Hit
TCTAAGTTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT570.1742799486332783No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.17122240567479974No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA560.17122240567479974No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG550.16816486271632117No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG540.1651073197578426No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC530.16204977679936403No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.14981960496544974No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA480.1467620620069712No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA470.14370451904849263No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA470.14370451904849263No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC470.14370451904849263No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC460.14064697609001406No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC460.14064697609001406No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA450.13758943313153552No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA440.13453189017305694No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT440.13453189017305694No Hit
TCTAAGTTGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC430.13147434721457835No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.1284168042560998No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA420.1284168042560998No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA410.12535926129762123No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG410.12535926129762123No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA410.12535926129762123No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA400.12230171833914266No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT400.12230171833914266No Hit
TCTAAGTTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT390.1192441753806641No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC390.1192441753806641No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT390.1192441753806641No Hit
ATTGCTGCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG390.1192441753806641No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG390.1192441753806641No Hit
TCTAAGTTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC380.11618663242218555No Hit
ATTGCTGCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA380.11618663242218555No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC380.11618663242218555No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.11618663242218555No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT370.11312908946370696No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC370.11312908946370696No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG360.1100715465052284No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT360.1100715465052284No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC360.1100715465052284No Hit
ATTGCTGCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA360.1100715465052284No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT350.10701400354674984No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10395646058827127No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10395646058827127No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT330.10089891762979271No Hit
ATTGCTGCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA330.10089891762979271No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT330.10089891762979271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)