FastQCFastQC Report
Sun 21 May 2023
EGAF00007847034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37055
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA17724.78208069086493No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA16424.43125084334098No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA15584.204560788017812No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA14533.9211982188638506No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT6511.7568479287545542No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT5651.5247604911617867No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5611.513965726622588No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5361.4464984482525973No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT4971.3412494939954123No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT4741.279179597895021No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4721.2737822156254217No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4301.1604371879638375No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT3891.049790851437053No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3630.9796248819322629No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3580.9661314262582648No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3460.9337471326406693No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3270.8824720010794764No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3190.8608824720010795No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT3140.8473890163270813No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT3080.8311968695182836No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2990.8069086493050871No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2910.78531912022669No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.7691269734178923No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT2850.7691269734178923No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2790.7529348266090946No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCT2770.7475374443394953No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2760.7448387532046957No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2760.7448387532046957No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2730.7367426798002968No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2650.7151531507218999No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCT2570.6935636216435029No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.6530832546215085No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2380.64228849008231No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2340.6314937255431116No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2340.6314937255431116No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2300.6206989610039131No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCATGCT2180.5883146673863177No Hit
TCTAAGTTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT2160.5829172851167184No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.5748212117123196No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2120.5721225205775199No Hit
TCTAAGTTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCT2090.564026447173121No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.5532316826339225No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2050.5532316826339225No Hit
TCTAAGTTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCT1980.5343408446903253No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.507353933342329No Hit
TCTAAGTTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCT1830.4938604776683309No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1780.4803670219943328No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.4614761840507354No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1670.4506814195115369No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1600.4317905815679396No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1590.4290918904331399No Hit
TCTAAGTTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCT1580.42639319929834035No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.41829712589394147No Hit
TCTAAGTTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCT1460.39400890568074487No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1450.3913102145459453No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1440.3886115234111456No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1430.385912832276346No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1380.37241937660234786No Hit
TCTAAGTTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCATGCT1360.3670219943327486No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.35892592092834974No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.35892592092834974No Hit
TCTAAGTTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCATGCT1310.3535285386587505No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1310.3535285386587505No Hit
TCTAAGTTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1290.34813115638915126No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1270.342733774119552No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1270.342733774119552No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1250.3373363918499528No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1210.32654162731075426No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1180.3184455539063554No Hit
TCTAAGTTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.3184455539063554No Hit
TCTAAGTTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1160.3130481716367562No Hit
TCTAAGTTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.30765078936715695No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.30765078936715695No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.30765078936715695No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.3049520982323573No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1120.3022534070975577No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.3022534070975577No Hit
TCTAAGTTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1110.29955471596275807No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1110.29955471596275807No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1100.2968560248279584No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1060.28606126028875994No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1050.2833625691539603No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1020.27526649574956147No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1020.27526649574956147No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1000.2698691134799622No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA990.26717042234516264No Hit
TCTAAGTTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT980.264471731210363No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA980.264471731210363No Hit
TCTAAGTTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT970.26177304007556335No Hit
TCTAAGTTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCT960.2590743489407637No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.2563756578059641No Hit
TCTAAGTTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT950.2563756578059641No Hit
TCTAAGTTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCT950.2563756578059641No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA920.24827958440156525No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC910.2455808932667656No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC900.24288220213196599No Hit
TCTAAGTTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCT890.2401835109971664No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA890.2401835109971664No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA880.23748481986236675No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.23478612872756713No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA860.2320874375927675No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA850.22938874645796786No Hit
TCTAAGTTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT850.22938874645796786No Hit
TCTAAGTTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT840.22669005532316827No Hit
TCTAAGTTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCT840.22669005532316827No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.2185939819187694No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.2185939819187694No Hit
TCTAAGTTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT790.21319659964917018No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.21319659964917018No Hit
TCTAAGTTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC790.21319659964917018No Hit
TCTAAGTTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC740.19970314397517205No Hit
TCTAAGTTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT740.19970314397517205No Hit
TCTAAGTTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT720.1943057617055728No Hit
TCTAAGTTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT720.1943057617055728No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA720.1943057617055728No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC720.1943057617055728No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.18890837943597355No Hit
TCTAAGTTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC700.18890837943597355No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC690.18620968830117393No Hit
TCTAAGTTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT680.1835109971663743No Hit
TCTAAGTTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT670.18081230603157467No Hit
TCTAAGTTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT670.18081230603157467No Hit
TCTAAGTTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT630.1700175414923762No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC630.1700175414923762No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.16462015922277695No Hit
TCTAAGTTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCT580.1565240858183781No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT570.15382539468357848No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.1484280124139792No Hit
TCTAAGTTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT540.1457293212791796No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA530.14303063014437997No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT520.14033193900958035No Hit
TCTAAGTTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT520.14033193900958035No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC520.14033193900958035No Hit
TCTAAGTTGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC510.13763324787478073No Hit
TCTAAGTTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT500.1349345567399811No Hit
TCTAAGTTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.1322358656051815No Hit
TCTAAGTTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT490.1322358656051815No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC490.1322358656051815No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA470.12683848333558226No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.12683848333558226No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.12683848333558226No Hit
TCTAAGTTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT470.12683848333558226No Hit
TCTAAGTTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.12144110106598299No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA450.12144110106598299No Hit
TCTAAGTTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT440.11874240993118337No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC440.11874240993118337No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT430.11604371879638375No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.11334502766158414No Hit
TCTAAGTTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.11334502766158414No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA420.11334502766158414No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT410.1106463365267845No Hit
TCTAAGTTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC410.1106463365267845No Hit
TCTAAGTTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.1079476453919849No Hit
TCTAAGTTGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC400.1079476453919849No Hit
TCTAAGTTGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT390.10524895425718526No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA390.10524895425718526No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.10524895425718526No Hit
TCTAAGTTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT380.10255026312238563No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA380.10255026312238563No Hit
TCTAAGTTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT380.10255026312238563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)