FastQCFastQC Report
Sun 21 May 2023
EGAF00007847059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22438
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6542.9146982797040732No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5062.2551029503520814No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4431.9743292628576523No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4321.9253052856760853No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4151.8495409573045727No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3781.6846421249665746No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3471.546483643818522No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3371.5019163918352796No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3301.4707193154470095No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3011.3414742846956056No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3001.3370175594972813No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3001.3370175594972813No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2981.3281041091006327No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2931.3058204831090114No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2841.265709956324093No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2721.212229253944202No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2701.2033158035475533No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2601.1587485515643106No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2391.065157322399501No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2341.0428736964078795No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2331.0384169712095552No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2291.020590070416258No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2281.016133345217934No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2251.002763169622961No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2170.9671093680363668No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.9136286656564756No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2010.8958017648631786No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2000.8913450396648542No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1940.8646046884749086No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1870.8334076120866387No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1840.8200374364916659No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1750.7799269097067475No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1630.7264462073268563No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1620.721989482128532No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.7130760317318834No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1600.7130760317318834No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1560.6952491309385863No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.6729655049469649No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1480.6595953293519922No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1440.6417684285586951No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1440.6417684285586951No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.6328549781620465No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1420.6328549781620465No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1410.6283982529637222No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1410.6283982529637222No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1400.6239415277653979No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1390.6194848025670737No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1390.6194848025670737No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1360.606114626972101No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1210.5392637489972368No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1200.5348070237989125No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1200.5348070237989125No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1150.5125233978072912No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.49915322221231834No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1090.48578304661734556No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.48578304661734556No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.48578304661734556No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1070.476869596220697No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1070.476869596220697No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1070.476869596220697No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1050.4679561458240485No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1040.4634994206257242No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1030.4590426954273999No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1020.4545859702290757No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.4545859702290757No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT890.39664854265086014No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT880.39219181745253584No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG870.3877350922542116No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA860.38327836705588736No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.38327836705588736No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC850.37882164185756306No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT840.37436491665923877No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA810.360994741064266No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT790.3520812906676174No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT790.3520812906676174No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC780.34762456546929316No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC780.34762456546929316No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA760.3387111150726446No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA720.32088421427934755No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA700.31197076388269895No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.3075140386843747No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT670.2986005882877262No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.2986005882877262No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT660.29414386308940194No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT660.29414386308940194No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA640.28523041269275334No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA640.28523041269275334No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA630.2807736874944291No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA630.2807736874944291No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT620.27631696229610486No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.27631696229610486No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT610.27186023709778057No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.27186023709778057No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC590.26294678670113203No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA590.26294678670113203No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC580.25849006150280773No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA560.24957661110615917No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA560.24957661110615917No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT550.24511988590783493No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG540.24066316070951066No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT530.23620643551118636No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT510.22729298511453785No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT490.2183795347178893No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT490.2183795347178893No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC480.21392280951956505No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT470.20946608432124073No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG460.20500935912291648No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT450.20055263392459222No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC440.19609590872626792No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA430.19163918352794368No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT430.19163918352794368No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA420.18718245832961938No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC410.18272573313129514No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC390.17381228273464658No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA390.17381228273464658No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT390.17381228273464658No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC390.17381228273464658No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC390.17381228273464658No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.1693555575363223No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.1693555575363223No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC380.1693555575363223No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.16489883233799804No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA370.16489883233799804No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA360.16044210713967377No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA360.16044210713967377No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT360.16044210713967377No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA360.16044210713967377No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA360.16044210713967377No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA350.15598538194134948No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT350.15598538194134948No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT350.15598538194134948No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT340.15152865674302524No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA340.15152865674302524No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT340.15152865674302524No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT340.15152865674302524No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT340.15152865674302524No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT330.14707193154470097No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT330.14707193154470097No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT320.14261520634637667No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC320.14261520634637667No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT320.14261520634637667No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT310.13815848114805243No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC300.13370175594972813No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA300.13370175594972813No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA300.13370175594972813No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT280.12478830555307958No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG280.12478830555307958No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT280.12478830555307958No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA280.12478830555307958No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT270.12033158035475533No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT270.12033158035475533No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA260.11587485515643105No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA240.10696140475978252No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC240.10696140475978252No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA240.10696140475978252No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT230.10250467956145824No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT230.10250467956145824No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA230.10250467956145824No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT230.10250467956145824No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT230.10250467956145824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)