FastQCFastQC Report
Sun 21 May 2023
EGAF00007847113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23253
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10634.571453145830646No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9153.9349761321119856No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5682.442695566163506No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4461.9180320818819077No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4451.9137315615189439No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4431.905130520793016No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4251.8277211542596654No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3941.694405023007784No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3391.4578764030447684No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3301.419171719778093No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3241.3933685976003096No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3171.3632649550595621No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3061.315959231066959No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2961.27295402743732No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2671.1482389369113661No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2661.1439384165484023No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2641.1353373758224745No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2611.1224358147335827No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2571.105233733281727No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2571.105233733281727No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2511.0794306111039436No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2501.0751300907409798No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2421.0407259278372682No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2381.0235238463854126No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.9719176020298457No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2190.9418139594890981No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.8300004300520364No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.8300004300520364No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1860.7998967875112889No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1840.791295746785361No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1820.7826947060594333No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1810.7783941856964692No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1800.7740936653335054No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1780.7654926246075775No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.7654926246075775No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1740.7482905431557219No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1700.7310884617038662No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1590.6837827377112631No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1560.6708811766223713No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1520.6536790951705156No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1490.6407775340816239No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.63647701371866No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1470.632176493355696No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1410.6063733711779126No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1400.6020728508149487No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1380.5934718100890208No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1360.5848707693630929No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1330.5719692082742012No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.5676686879112373No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1300.5590676471853094No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1270.5461660860964177No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1130.48595880101492284No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.4472541177482476No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1030.4429535973852836No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.4386530770223197No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1020.4386530770223197No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.4343525566593558No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1010.4343525566593558No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.4300520362963919No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.4171504752075001No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT960.4128499548445362No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.4085494344815723No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT950.4085494344815723No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT920.3956478733926805No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA880.37844579194082484No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.3741452715778609No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT830.3569431901260052No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT820.3526426697630413No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG790.3397411086741496No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT740.31823850685933No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT740.31823850685933No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT740.31823850685933No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.31823850685933No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA730.31393798649636606No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.31393798649636606No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT710.30533694577043824No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA710.30533694577043824No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT690.2967359050445104No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG690.2967359050445104No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC690.2967359050445104No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA660.28383434395561863No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.28383434395561863No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC640.2752333032296908No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT630.27093278286672684No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC630.27093278286672684No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT620.26663226250376293No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.2623317421407991No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA610.2623317421407991No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT610.2623317421407991No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG600.2580312217778351No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC570.24512966068894337No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT540.23222809960005159No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT540.23222809960005159No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA520.2236270588741238No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.2236270588741238No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT520.2236270588741238No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.21932653851115985No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA490.21072549778523203No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA490.21072549778523203No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT490.21072549778523203No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT490.21072549778523203No Hit
TCTACTACTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT480.2064249774222681No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA470.20212445705930418No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA460.19782393669634024No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT460.19782393669634024No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT460.19782393669634024No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT460.19782393669634024No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT460.19782393669634024No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT450.19352341633337636No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC450.19352341633337636No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.18922289597041242No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA430.1849223756074485No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT420.18062185524448457No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA420.18062185524448457No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT410.17632133488152066No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.17202081451855675No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA390.16772029415559284No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT370.159119253429665No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT360.15481873306670108No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.15481873306670108No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.15481873306670108No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA360.15481873306670108No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA350.15051821270373716No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC320.1376166516148454No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA310.13331613125188146No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA310.13331613125188146No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT310.13331613125188146No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT310.13331613125188146No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA310.13331613125188146No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA310.13331613125188146No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA290.12471509052595364No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC290.12471509052595364No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT290.12471509052595364No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC290.12471509052595364No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT290.12471509052595364No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT280.12041457016298972No Hit
TCTACTACTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT280.12041457016298972No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT270.11611404980002579No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT270.11611404980002579No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC270.11611404980002579No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT260.1118135294370619No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT260.1118135294370619No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC260.1118135294370619No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC250.10751300907409797No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT250.10751300907409797No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG250.10751300907409797No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA250.10751300907409797No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT250.10751300907409797No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA250.10751300907409797No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC240.10321248871113405No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA240.10321248871113405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)